Abhd5 Antibody

Novus Biologicals | Catalog # NB100-57850

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Abhd5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-FPERPDLADQDR corresponding to internal region according to NP_057090.2.

Specificity

This antibody is not expected to cross-react with ABHD4.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Abhd5 Antibody

Western Blot: Abhd5 Antibody [NB100-57850]

Western Blot: Abhd5 Antibody [NB100-57850]

Western Blot: Abhd5 Antibody [NB100-57850] - Mesenchymal stem cells differentiated into adipocytes. Antibody at 1:1000 in 5% BSA. Clear, strong band at expected molecular weight. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Abhd5 Antibody [NB100-57850]

Immunocytochemistry/ Immunofluorescence: Abhd5 Antibody [NB100-57850]

Immunocytochemistry/Immunofluorescence: Abhd5 Antibody [NB100-57850] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and vesicle/ cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850]

Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850]

Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Abhd5 Antibody [NB100-57850]

Western Blot: Abhd5 Antibody [NB100-57850]

Western Blot: Abhd5 Antibody [NB100-57850] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer), antibody at 0.2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850]

Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850]

Immunohistochemistry-Paraffin: Abhd5 Antibody [NB100-57850] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Abhd5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

3.75 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.2 - 0.6 ug/mL
Application Notes
Western blot: Approx 40kDa band observed in lysates of cell line NIH3T3 (calculated MW of 39.1kDa according to NP_057090.2). Primary incubation was 1 hour. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Pancreas and Kidney.

Reviewed Applications

Read 3 reviews rated 5 using NB100-57850 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Abhd5

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Alternate Names

abhydrolase domain containing 5, Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5, CDS, CGI58, EC 2.3.1.51, IECN2, Lipid droplet-binding protein CGI-58, MGC8731, NCIE2CGI-58

Entrez Gene IDs

51099 (Human); 67469 (Mouse); 316122 (Rat)

Gene Symbol

ABHD5

UniProt

Q8WTS1 (Human)

Additional Abhd5 Products

Product Documents for Abhd5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Abhd5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Abhd5 Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Abhd5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mesenchymal stem cells differentiated into adipocytes
    Species: Mouse
    Verified Customer | Posted 12/06/2018
    1:1000 in 5% BSA. Clear, strong band at expected molecular weight.
    PVDF membrane, 10% gel 180V 1hr; semi-dry transfer
    Abhd5 Antibody NB100-57850
  • Abhd5 Antibody
    Name: Johana Lambert
    Application: Western Blot
    Sample Tested: Mesenchymal stem cells differentiated into adipocytes
    Species: Mouse
    Verified Customer | Posted 11/16/2018
    Used 20 ug protein. Blocked 1 hour in 5% BSA. Primary incubated at 1:1000 in 5% BSA (TBST). Incubated primary overnight at 4 degrees C. Clear band between 37-50 kDa. Faint band above 50 kDa.
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 01/15/2014

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Protocols

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