Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)

R&D Systems | Catalog # S-310

R&D Systems
Discontinued Product
S-310 has been discontinued. View all Proteasome Substrates products.

Key Product Details

Source

Synthetic

Conjugate

7-Amino-4-Methyl Coumarin
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Formulation, Preparation, and Storage

S-310
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)

For research use only

Related Research Areas

Citations for Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)

Customer Reviews for Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC) (2)

3 out of 5
2 Customer Ratings
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  • Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)
    Name: TAKASHI SHIMADA
    Verified Customer | Posted 05/02/2020
    S-310 hydrolysis confirmation by LCMS-SRM S-310: 1 uM i20S: 15 nM PA28a: 150 nM Reaction: 37 °C for 5 h 1:S-310 Ac-PAL-AMC (499.25>211.10) 2:Free AMC (176.25>120.00) 3:S-310 Ac-PAL-COOH (342.20>211.10)
    Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC) S-310
  • Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)
    Name: TAKASHI SHIMADA
    Verified Customer | Posted 05/02/2020
    Ordinate: fluorescent count, Em 345/Ex 445 Em band path: 9 nm Ex band path: 20 nm Horizontal: time [min] Temp: 37 °C S-310: 5, 25, 50, and 100 uM i20S: 100 ng per assay (100 uL) The linearity of the kinetics was confirmed, and the fluorescence intensity increased in a concentration-dependent manner. But it is considered that the saturated level has not been reached using this condition. Probably it could be a low amount of i20S. Compared with S-320, S-310 has slightly higher level of intrinsic fluorescence.
    Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC) S-310

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