Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)

Catalog # Availability Size / Price Qty
S-310
Product Details
Citations (1)
FAQs
Reviews (2)

Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC) Summary

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-Species

Product Datasheets

Citation for Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth
    Authors: MJ Lee, Z Miller, JE Park, D Bhattarai, W Lee, KB Kim
    Sci Rep, 2019;9(1):4089.  2019

FAQs

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Reviews for Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)

Average Rating: 3 (Based on 2 Reviews)

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Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)
By Anonymous on 05/02/2020
Application:

S-310 hydrolysis confirmation by LCMS-SRM
S-310: 1 uM
i20S: 15 nM
PA28a: 150 nM
Reaction: 37 °C for 5 h
1:S-310 Ac-PAL-AMC (499.25>211.10)
2:Free AMC (176.25>120.00)
3:S-310 Ac-PAL-COOH (342.20>211.10)


Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)
By Anonymous on 05/02/2020
Application:

Ordinate: fluorescent count, Em 345/Ex 445
Em band path: 9 nm
Ex band path: 20 nm
Horizontal: time [min]
Temp: 37 °C
S-310: 5, 25, 50, and 100 uM
i20S: 100 ng per assay (100 uL)
The linearity of the kinetics was confirmed, and the fluorescence intensity increased in a concentration-dependent manner. But it is considered that the saturated level has not been reached using this condition. Probably it could be a low amount of i20S.
Compared with S-320, S-310 has slightly higher level of intrinsic fluorescence.