Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC) Summary
Citation for Ac-Pro-Ala-Leu-AMC (Ac-PAL-AMC)
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1 Citation: Showing 1 - 1
H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth
Authors: MJ Lee, Z Miller, JE Park, D Bhattarai, W Lee, KB Kim
Sci Rep, 2019;9(1):4089. 2019
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S-310 hydrolysis confirmation by LCMS-SRM
S-310: 1 uM
i20S: 15 nM
PA28a: 150 nM
Reaction: 37 °C for 5 h
1:S-310 Ac-PAL-AMC (499.25>211.10)
2:Free AMC (176.25>120.00)
3:S-310 Ac-PAL-COOH (342.20>211.10)
Ordinate: fluorescent count, Em 345/Ex 445
Em band path: 9 nm
Ex band path: 20 nm
Horizontal: time [min]
Temp: 37 °C
S-310: 5, 25, 50, and 100 uM
i20S: 100 ng per assay (100 uL)
The linearity of the kinetics was confirmed, and the fluorescence intensity increased in a concentration-dependent manner. But it is considered that the saturated level has not been reached using this condition. Probably it could be a low amount of i20S.
Compared with S-320, S-310 has slightly higher level of intrinsic fluorescence.