ACAA2 Antibody (12F4) - BSA Free

Novus Biologicals | Catalog # NBP3-26536

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 12F4 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human ACAA2 [UniProt P42765]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACAA2 Antibody (12F4) - BSA Free

ACAA2 Antibody (12F4)

Immunocytochemistry/Immunofluorescence: ACAA2 Antibody (12F4) [NBP3-26536] -

Immunocytochemistry/Immunofluorescence: ACAA2 Antibody (12F4) [NBP3-26536] - Staining of Hela cell with ACAA2 Antibody (12F4) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 570-conjugated Goat Anti-Rabbit IgG (H+L).
ACAA2 Antibody (12F4)

Immunocytochemistry/Immunofluorescence: ACAA2 Antibody (12F4) [NBP3-26536]

Immunocytochemistry/Immunofluorescence: ACAA2 Antibody (12F4) [NBP3-26536] -Immunofluorescence staining of Hela cell with ACAA2 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 570-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ACAA2 Antibody (12F4)

Immunohistochemistry: ACAA2 Antibody (12F4) [NBP3-26536]

Immunohistochemistry: ACAA2 Antibody (12F4) [NBP3-26536] -IHC image of ACAA2 Antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Applications for ACAA2 Antibody (12F4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ACAA2

The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.

Alternate Names

3-ketoacyl-CoA thiolase, mitochondrial, Acetyl-CoA acyltransferase, acetyl-CoA acyltransferase 2, acetyl-Coenzyme A acyltransferase 2, beta ketothiolase, beta-ketothiolase, DSAEC, EC 2.3.1, EC 2.3.1.16, FLJ35992, FLJ95265, Mitochondrial 3-oxoacyl-CoA thiolase, mitochondrial 3-oxoacyl-Coenzyme A thiolase, T1

Gene Symbol

ACAA2

Additional ACAA2 Products

Product Documents for ACAA2 Antibody (12F4) - BSA Free

Certificate of Analysis

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Product Specific Notices for ACAA2 Antibody (12F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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