ACADM Antibody (1E5G10)

Novus Biologicals | Catalog # NBP3-16437

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1E5G10 expressed in HEK293
View all ACADM Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ACADM (P11310). WYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEAT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACADM Antibody (1E5G10)

Western Blot: ACADM Antibody (1E5G10) [NBP3-16437]

Western Blot: ACADM Antibody (1E5G10) [NBP3-16437]

Western Blot: ACADM Antibody (1E5G10) [NBP3-16437] - Western blot analysis of extracts of various cell lines, using ACADM Rabbit mAb (NBP3-16437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: ACADM Antibody (1E5G10) [NBP3-16437]

Immunocytochemistry/ Immunofluorescence: ACADM Antibody (1E5G10) [NBP3-16437]

Immunocytochemistry/Immunofluorescence: ACADM Antibody (1E5G10) [NBP3-16437] - Immunofluorescence analysis of NIH-3T3 cells using ACADM Rabbit mAb (NBP3-16437) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ACADM Antibody (1E5G10) [NBP3-16437]

Immunohistochemistry-Paraffin: ACADM Antibody (1E5G10) [NBP3-16437]

Immunohistochemistry-Paraffin: ACADM Antibody (1E5G10) [NBP3-16437] - Immunohistochemistry of paraffin-embedded mouse kidney using ACADM Rabbit mAb (NBP3-16437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ACADM Antibody (1E5G10) [NBP3-16437]

Immunohistochemistry-Paraffin: ACADM Antibody (1E5G10) [NBP3-16437]

Immunohistochemistry-Paraffin: ACADM Antibody (1E5G10) [NBP3-16437] - Immunohistochemistry of paraffin-embedded human colon using ACADM Rabbit mAb (NBP3-16437) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for ACADM Antibody (1E5G10)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunohistochemistry

1:200 - 1:800

Immunohistochemistry-Paraffin

1:200 - 1:800

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACADM

ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.

Alternate Names

ACAD1, acyl-CoA dehydrogenase, C-4 to C-12 straight chain, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain, EC 1.3.99, EC 1.3.99.3, FLJ18227, FLJ99884, MCADFLJ93013, MCADH, medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene Symbol

ACADM

Additional ACADM Products

Product Documents for ACADM Antibody (1E5G10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ACADM Antibody (1E5G10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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