ACAT1 Antibody

Novus Biologicals | Catalog # NBP1-46140

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ACAT1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DEEYKRVDFSKVPK corresponding to internal region according to NP_000010.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Goat ACAT1 Antibody (NBP1-46140) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-ACAT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ACAT1 Antibody

ACAT1 Antibody

Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] -

Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-46140] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
ACAT1 Antibody

Western Blot: ACAT1 Antibody [NBP1-46140] -

Western Blot: ACAT1 Antibody [NBP1-46140] - (0.01ug/ml) staining of Human (A), and (0.1ug/ml) Mouse (B) and Rat (C), Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
ACAT1 Antibody

Flow Cytometry: ACAT1 Antibody [NBP1-46140] -

Flow Cytometry: ACAT1 Antibody [NBP1-46140] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
ACAT1 Antibody

Western Blot: ACAT1 Antibody [NBP1-46140] -

Western Blot: ACAT1 Antibody [NBP1-46140] - (0.5ug/ml) staining of KNRK (A) and MCF7 (B), and (0.1ug/ml) HepG2 (C), Caco-2 (D), HEK293 (E), and NIH3T3 (F) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for ACAT1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:4000

Western Blot

0.01-0.5 ug/ml
Application Notes
WB: Approx. 40kDa band observed in lysates of cell lines CAC02, HEK293, HepG2, MCF7, NIH3T3 and KNRK and approx. 38-40kDa in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kDa according to Human NP_000010.1, 44.81kDa according to Mouse NP_659033.1 and 44.7kDa according to Rat NP_058771.2 ). Primary incubation 1 hour at room temperature.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACAT1

ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.

Long Name

Acetyl-CoA Acetyltransferase 1

Alternate Names

THIL

Entrez Gene IDs

38 (Human); 110446 (Mouse); 25014 (Rat)

Gene Symbol

ACAT1

UniProt

P24752

Additional ACAT1 Products

Product Documents for ACAT1 Antibody

Certificate of Analysis

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Product Specific Notices for ACAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bioactive Lipids and Receptors

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Protocols

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FAQs

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