ACE-2 Antibody (CL4013) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44114

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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4013

Format

Azide and BSA Free
View all ACE-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q9BYF1, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ACE-2 Antibody (CL4013) - Azide and BSA Free

ACE-2 Antibody (CL4013) - Azide and BSA Free Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Analysis in human kidney and tonsil tissues using NBP3-44114 antibody. Corresponding ACE-2 RNA-seq data are presented for the same tissues.
ACE-2 Antibody (CL4013) - Azide and BSA Free Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Staining of human kidney shows strong positivity in apical membrane in cells in proximal tubules.
ACE-2 Antibody (CL4013) - Azide and BSA Free Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Staining of human small intestine shows strong positivity in apical membrane in glandular cells.
ACE-2 Antibody (CL4013) - Azide and BSA Free Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
ACE-2 Antibody (CL4013) - Azide and BSA Free Western Blot: ACE-2 Antibody [NBP3-44114]

Western Blot: ACE-2 Antibody [NBP3-44114]

Analysis in human kidney tissue.
ACE-2 Antibody (CL4013) - Azide and BSA Free Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP3-44114]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Applications for ACE-2 Antibody (CL4013) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE-2

Angiotensin I Converting Enzyme 2 (ACE2), also known as ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family (theoretical molecular weight 92 kDa). The predicted mouse ACE2 protein sequence consists of 798 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. Mouse ACE2 is 40% homologous in amino acid identity to the N- and C-terminal domains of mouse somatic ACE. Enzymatically, ACE2 converts the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts Ang I to Ang II.

Genetic data from Drosophila, mice and rats show that the ACE2 protein is an essential regulator of heart function in vivo. ACE2 mRNA is found at high levels in testis, kidney and heart with moderate levels in colon, small intestine, and ovary. The ACE2 protein contributes to organ function through the renin-angiotensin system, playing a central role in vascular, renal, and myocardial physiology.

Virology research has demonstrated that the severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein binds to its functional receptor, ACE2 (1). Vimentin is a type III intermediate filament protein expressed in mesenchymal cells that helps comprise the cytoskeleton in all animal cells. Studies have demonstrated that vimentin allows cell binding that allows the uptake of SARS-CoV spike protein into a host (2). This direct interaction of SARS-CoV with vimentin has been identified as an entry mechanism for the virus into a host, mediated by the SARS-CoV receptor ACE2 (1,2). It has been shown that ACE2 is the SARS-CoV-2 receptor required for cell entry and plays a physiological role in the replication of SARS-CoV in an infected host (1). Studies using human ACE2 antibody demonstrated a blockade of SARS-CoV-2 and ACE2 interaction, indicating an important physiological component of viral transmission and potential anti-viral therapeutic strategies (3).

References

1. Li, W., Moore, M. J., Vasilieva, N., Sui, J., Wong, S. K., Berne, M. A.,... Farzan, M. (2003). Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426(6965), 450-454. doi:10.1038/nature02145

2. Yu YT, Chien SC, Chen IY, Lai CT, Tsay YG, Chang SC, Chang MF. (2016) Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci. doi: 10.1186/s12929-016-0234-7

3. Hoffmann, M., Kleine-Weber, H., Schroeder, S., Kruger, N., Herrler, T., Erichsen, S.,... Pohlmann, S. (2020). SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. doi:10.1016/j.cell.2020.02.052

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Gene Symbol

ACE2

Additional ACE-2 Products

Product Documents for ACE-2 Antibody (CL4013) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ACE-2 Antibody (CL4013) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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