Acetylcholinesterase/ACHE Antibody (HL1102) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13720

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1102 expressed in HEK293

Format

Azide and BSA Free
View all Acetylcholinesterase/ACHE Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Acetylcholinesterase/ACHE. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Acetylcholinesterase/ACHE Antibody (HL1102) - Azide and BSA Free

Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720]

Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720]

Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Acetylcholinesterase/ACHE antibody [HL1102] detects Acetylcholinesterase/ACHE protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720]

Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720]

Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Acetylcholinesterase/ACHE Antibody (HL1102)

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] -

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Acetylcholinesterase/ACHE antibody [HL1102] detects Acetylcholinesterase/ACHE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Acetylcholinesterase/ACHE Antibody (HL1102)

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] -

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Acetylcholinesterase/ACHE antibody [HL1102] detects Acetylcholinesterase/ACHE protein at dendrite by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Acetylcholinesterase/ACHE Antibody (HL1102)

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] -

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Acetylcholinesterase/ACHE antibody [HL1102] detects Acetylcholinesterase/ACHE protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Scale bar= 10um.
Acetylcholinesterase/ACHE Antibody (HL1102)

Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] -

Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Acetylcholinesterase/ACHE antibody [HL1102] (NBP3-13720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Acetylcholinesterase/ACHE Antibody (HL1102) - Azide and BSA Free

Western Blot: Acetylcholinesterase/ACHE Antibody (HL1102) [NBP3-13720] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (NBP3-13720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Acetylcholinesterase/ACHE Antibody (HL1102) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Acetylcholinesterase/ACHE

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Alternate Names

ACHE, ARACHE, N-ACHE

Gene Symbol

ACHE

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Antibody (HL1102) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Acetylcholinesterase/ACHE Antibody (HL1102) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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