Acetylcholinesterase/ACHE Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31329

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (94%), Feline (95%), Guinea Pig (92%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Acetylcholinesterase/ACHE Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Acetylcholinesterase/ACHE. The exact sequence is proprietary.

Reactivity Notes

Cat (95%).

Localization

Cell junction, synapse, Secreted, Cell membrane, Peripheral membrane protein, Isoform T: Nucleus, Isoform H: Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, Cell junction, synapse, Secreted, Cell membrane, Isoform T: Nucleus, Isof

Marker

Early Neuronal Development Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Acetylcholinesterase/ACHE Antibody - BSA Free

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse brain. AChE stained by AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Paraformaldehyde-fixed HeLa, using AChE antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse colon. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat fore brain. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329]

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat brain. AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Acetylcholinesterase/ACHE Antibody

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] -

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Acetylcholinesterase/ACHE Antibody

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] -

Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Acetylcholinesterase/ACHE Antibody

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] -

Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Acetylcholinesterase/ACHE Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329] -

Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.
Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Acetylcholinesterase/ACHE Antibody - BSA Free

Immunohistochemistry-Frozen: Acetylcholinesterase/ACHE Antibody [NBP1-31329] -

Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Acetylcholinesterase/ACHE protein stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Acetylcholinesterase/ACHE Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Acetylcholinesterase/ACHE

Acetylcholinesterase (AChE) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa.

Alternate Names

ACHE, ARACHE, N-ACHE

Entrez Gene IDs

43 (Human)

Gene Symbol

ACHE

UniProt

P22303 (Human)

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Acetylcholinesterase/ACHE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Acetylcholinesterase/ACHE Antibody - BSA Free

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  • Q: We recently purchased your Acetylcholinesterase/ACHE Antibody (NBP1-31329) and Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (NB7160) for western blot. What buffer do you recommend for diluting these particular antibodies and what is the recommended blocking buffer?

    A:

    Acetylcholinesterase/ACHE Antibody #NBP1-31329 is already in PBS and 20% glycerol.  Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] #NB7160 is in PBS and 0.2% BSA.  You can use PBS for diluting either one of them. We do not have a specific protocol for these antibodies. Our standard in house blocking buffer is TBST with 5% non-fat dry milk. You may refer to our general protocol for any more details or tips.

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