ACF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90270

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RGHRESALKETLLQEKSRICAQLARFSEEKFHFSDKPQPDSKPTYSRGRSSNAYDPSQMCAEKQLELRLRDFLLDIEDRIYQGTLGAIKVTDRHIWRSALESGRYELLSEENKENGIIKTVNEDVEEMEIDEQTK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%). Reactivity reported in scientific literature (PMID: 24244200)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACF1 Antibody - BSA Free

ACF1 Antibody - BSA Free Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Analysis in human testis and pancreas tissues using NBP1-90270 antibody. Corresponding BAZ1A RNA-seq data are presented for the same tissues.
ACF1 Antibody - BSA Free Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
ACF1 Antibody - BSA Free Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
ACF1 Antibody - BSA Free Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Staining of human tonsil shows strong nuclear positivity in germinal center cells.
ACF1 Antibody - BSA Free Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Immunohistochemistry: ACF1 Antibody - BSA Free [NBP1-90270]

Staining of human placenta shows strong nuclear positivity in syncytiotrophoblast.

Applications for ACF1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACF1

ACF1/BAZ1A is a subunit of the ACF chromatin-remodeling complex and the ISWI CHRAC remodeling complex. In the ACF complex, ACF1/BAZ1A functions to influence the nucleosome remodeling activity of the SNF2h ATPase. As part of the CHRAC complex, ACF1/BAZ1A may function to target CHRAC to heterochromatin. ACF1 has also been shown to interact with the nuclear receptor corepressor protein (N-CoR) to facilitate the repression of transcription by unliganded nuclear receptors. Alternate names for ACF1/BAZ1A include ATP-utilizing chromatin assembly and remodeling factor1, hACF1, bromodomain adjacent to zinc finger domain protein 1A, Williams syndrome transcription factor-related chromatin-remodeling factor 180, WCRF180, hWALp1, and CHRAC subunit ACF1.

Alternate Names

ACF1hWALp1, ATP-dependent chromatin remodeling protein, ATP-dependent chromatin-remodeling protein, ATP-utilizing chromatin assembly and remodeling factor 1, bromodomain adjacent to zinc finger domain protein 1A, bromodomain adjacent to zinc finger domain, 1A, CHRAC subunit ACF1, hACF1FLJ14383, WALp1, WCRF180DKFZp586E0518, Williams syndrome transcription factor-related chromatin-remodeling factor 180

Gene Symbol

BAZ1A

Additional ACF1 Products

Product Documents for ACF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ACF1 Antibody - BSA Free

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Protocols

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