Acidic Calponin Antibody

Novus Biologicals | Catalog # NBP1-51972

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-HGEYQDDYPRDYQYS corresponding to C-Terminus according to NP_001830.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Acidic Calponin Antibody

Western Blot: Acidic Calponin Antibody [NBP1-51972]

Western Blot: Acidic Calponin Antibody [NBP1-51972]

Western Blot: Acidic Calponin Antibody [NBP1-51972] - NBP1-51972 (0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP1-51972]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP1-51972]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP1-51972] - (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Acidic Calponin Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.2 - 0.6 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human duodenum, heart and kidney lysates (calculated MW of 36.4 kDa band according to NP_001830.1). Primary incubation was 1 hour.
IHC: Paraffin embedded Human Lung (Bronchus).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acidic Calponin

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction.

Alternate Names

calponin 3, acidic, Calponin, acidic isoform, calponin-3, dJ639P13.2.2 (acidic calponin 3)

Entrez Gene IDs

1266 (Human)

Gene Symbol

CNN3

UniProt

Additional Acidic Calponin Products

Product Documents for Acidic Calponin Antibody

Certificate of Analysis

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Product Specific Notices for Acidic Calponin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Acidic Calponin Antibody

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Protocols

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