ACRBP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85407

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACRBP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AKRVLCSQPVSILSPNTLKEIEASAEVSPTTMTSPISPHFTVTERQTFQPWPERLSNNVEELLQSSLSLGGQEQAPEHKQEQGVEHRQEPTQEHKQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACRBP Antibody - BSA Free

Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407]

Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407]

Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407] - Staining in human testis and endometrium tissues using anti-ACRBP antibody. Corresponding ACRBP RNA-seq data are presented for the same tissues.
Western Blot: ACRBP Antibody [NBP1-85407]

Western Blot: ACRBP Antibody [NBP1-85407]

Western Blot: ACRBP Antibody [NBP1-85407] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: ACRBP Antibody [NBP1-85407]

Immunocytochemistry/ Immunofluorescence: ACRBP Antibody [NBP1-85407]

Immunocytochemistry/Immunofluorescence: ACRBP Antibody [NBP1-85407] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407]

Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407]

Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407]

Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407]

Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407] - Staining of human testis shows high expression.

Applications for ACRBP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC, Retrieval method: HIER pH6, ICC/IF, Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACRBP

ACRBP is encoded by this gene is similar to proacrosin binding protein sp32 precursor found in mouse, guinea pig, and pig. This protein is located in the sperm acrosome and is thought to function as a binding protein to proacrosin for packaging and condensation of the acrosin zymogen in the acrosomal matrix. This protein is a member of the cancer/testis family of antigens and it is found to be immunogenic. In normal tissues, this mRNA is expressed only in testis, whereas it is detected in a range of different tumor types such as bladder, breast, lung, liver, and colon. [provided by RefSeq]

Alternate Names

acrosin binding protein, acrosin-binding protein, Cancer/testis antigen 23, Cancer/testis antigen OY-TES-1, CT23FLJ51160, OY-TES-1, proacrosin binding protein sp32, Proacrosin-binding protein sp32, SP32

Entrez Gene IDs

84519 (Human)

Gene Symbol

ACRBP

UniProt

Q8NEB7 (Human)

Additional ACRBP Products

Product Documents for ACRBP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACRBP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ACRBP Antibody - BSA Free

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Protocols

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