Actin Antibody (3U6B8)

Novus Biologicals | Catalog # NBP3-16100

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3U6B8 expressed in HEK293
View all Actin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 278-377 of human alpha-Actin-1 (ACTA1) (P68133). ETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Actin Antibody (3U6B8)

Western Blot: Actin Antibody (3U6B8) [NBP3-16100]

Western Blot: Actin Antibody (3U6B8) [NBP3-16100]

Western Blot: Actin Antibody (3U6B8) [NBP3-16100] - Western blot analysis of extracts of various cell lines, using Actin Rabbit mAb (NBP3-16100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Actin Antibody (3U6B8)

Immunohistochemistry: Actin Antibody (3U6B8) [NBP3-16100] -

Immunohistochemistry: Actin Antibody (3U6B8) [NBP3-16100] - Immunohistochemistry analysis of paraffin-embedded Human kidney using alpha-Actin-1 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Actin Antibody (3U6B8)

Immunocytochemistry/ Immunofluorescence: Actin Antibody (3U6B8) [NBP3-16100] -

Immunocytochemistry/ Immunofluorescence: Actin Antibody (3U6B8) [NBP3-16100] - Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using alpha-Actin-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Actin Antibody (3U6B8)

Immunocytochemistry/ Immunofluorescence: Actin Antibody (3U6B8) [NBP3-16100] -

Immunocytochemistry/ Immunofluorescence: Actin Antibody (3U6B8) [NBP3-16100] - Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using alpha-Actin-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Actin Antibody (3U6B8)

Immunohistochemistry: Actin Antibody (3U6B8) [NBP3-16100] -

Immunohistochemistry: Actin Antibody (3U6B8) [NBP3-16100] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using alpha-Actin-1 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Actin Antibody (3U6B8)

Immunohistochemistry: Actin Antibody (3U6B8) [NBP3-16100] -

Immunohistochemistry: Actin Antibody (3U6B8) [NBP3-16100] - Immunohistochemistry analysis of paraffin-embedded Human spleen using alpha-Actin-1 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for Actin Antibody (3U6B8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Actin

Actin is a 43kDa filament protien that is ubiquitously expressed by all eukaryotic cells. This wide expression makes Actin an ideal "housekeeping gene", which may even account for up to 50% of total cellular protein. Actin microfilaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Actin antibodies are most commonly used as controls for protein standardization or in cytoskeletan or cell cycle research.

Alternate Names

ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3

Gene Symbol

ACTA1

Additional Actin Products

Product Documents for Actin Antibody (3U6B8)

Certificate of Analysis

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Product Specific Notices for Actin Antibody (3U6B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Actin Antibody (3U6B8)

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  • Q: I am interested in buying an Actin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?

    A:

    I would recommend the Actin Antibody (mAbGEa) catalog # NB100-74340. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

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