Acylglycerol Kinase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32163

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AGK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Acylglycerol Kinase. The exact sequence is proprietary.

Localization

Mitochondrion membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Acylglycerol Kinase Antibody - BSA Free

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163]

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163]

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] - Sample(30 ug of whole cell lysate) A:A431 B:H1299 10% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Acylglycerol Kinase Antibody [NBP1-32163]

Immunocytochemistry/ Immunofluorescence: Acylglycerol Kinase Antibody [NBP1-32163]

Immunocytochemistry/Immunofluorescence: Acylglycerol Kinase Antibody [NBP1-32163] - Methanol-fixed Hep3B, using Acylglycerol kinase (NBP1-32163) antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163] - Acylglycerol kinase antibody detects Acylglycerol kinase protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. Acylglycerol kinase antibody diluted at 1:500.
Western Blot: Acylglycerol Kinase Antibody [NBP1-32163]

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163]

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] - 50 ug mouse heart lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.
Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163] - Acylglycerol kinase antibody detects Acylglycerol kinase protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Acylglycerol kinase antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP1-32163] - Acylglycerol kinase antibody detects Acylglycerol kinase protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Acylglycerol kinase antibody diluted at 1:500.
Acylglycerol Kinase Antibody

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] -

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] -Mouse tissue extract (30ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.
Acylglycerol Kinase Antibody

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] -

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Acylglycerol Kinase Antibody

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] -

Western Blot: Acylglycerol Kinase Antibody [NBP1-32163] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Acylglycerol kinase antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Acylglycerol Kinase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AGK

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth

Long Name

Acylglycerol Kinase

Alternate Names

FLJ10842, MULK

Entrez Gene IDs

55750 (Human)

Gene Symbol

AGK

UniProt

Q53H12 (Human)

Additional AGK Products

Product Documents for Acylglycerol Kinase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Acylglycerol Kinase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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FAQs

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