ADAR Antibody (CL0176) - BSA Free

Novus Biologicals | Catalog # NBP3-05500

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0176

Format

BSA Free
View all ADAR Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P55265, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ADAR Antibody (CL0176) - BSA Free

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-05500 antibody. Corresponding ADAR RNA-seq data are presented for the same tissues.
Western Blot: ADAR Antibody (CL0176) [NBP3-05500]

Western Blot: ADAR Antibody (CL0176) [NBP3-05500]

Western Blot: ADAR Antibody (CL0176) [NBP3-05500] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADAR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ADAR Antibody (CL0176) [NBP3-05500]

Immunocytochemistry/ Immunofluorescence: ADAR Antibody (CL0176) [NBP3-05500]

Immunocytochemistry/Immunofluorescence: ADAR Antibody (CL0176) [NBP3-05500] - Staining of A549 cells using the Anti-ADAR monoclonal antibody, showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human cerebral cortex shows nuclear positivity.
Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human testis shows nuclear positivity.
Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500]

Immunohistochemistry-Paraffin: ADAR Antibody (CL0176) [NBP3-05500] - Staining of human kidney shows strong nuclear positivity in cells in tubules.
ADAR Antibody (CL0176) - BSA Free

Western Blot: ADAR Antibody (CL0176) - BSA Free [NBP3-05500] -

ADAR1 knockdown results in dsRNA increases, type I interferon signaling and pro-inflammatory protein expression. (A) Immunoblots for MDA5 and RIG-I following ADAR1 knockdown. Unpaired t-test; n = 6/condition. (B) Representative images and relative J2 (dsRNA antibody) immunofluorescence signal showing dsRNA accumulation following ADAR1 knockdown. (C) Representative immunofluorescence images showing co-localization of dsRNA (J2) and MDA5 after transfection with ADAR1 siRNA. (D) Summary immunoblot data for ICAM-1, TNF-alpha, pIRF3, pNF-kappa B, and (E) representative images following ADAR1 knockdown. (F) Concentrations of CXCL10 and IFN beta following ADAR1 siRNA knockdown. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 in unpaired t-test; n = 9/condition. Error bars represent SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38035265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ADAR Antibody (CL0176) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF fixation permeabilization use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAR

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Double-stranded RNA-specific adenosine deaminase

Alternate Names

ADAR1, DRADA, DSRAD, EC 3.5.4.37, G1P1, IFI-4, IFI4, K88DSRBP, p136

Gene Symbol

ADAR

Additional ADAR Products

Product Documents for ADAR Antibody (CL0176) - BSA Free

Certificate of Analysis

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Product Specific Notices for ADAR Antibody (CL0176) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ADAR Antibody (CL0176) - BSA Free

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Protocols

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