ADAR Antibody

Novus Biologicals | Catalog # NBP1-90242

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ADAR Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:SDNQPEGMISESLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEA

Reactivity Notes

Mouse (86%), Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ADAR Antibody

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining in human testis and skeletal muscle tissues using NBP1-90242 antibody. Corresponding ADAR RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining of human cerebellum shows strong nuclear positivity in all layers.
Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody [NBP1-90242] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
ADAR Antibody Immunohistochemistry-Paraffin: ADAR Antibody Antibody [NBP1-90242]

Immunohistochemistry-Paraffin: ADAR Antibody Antibody [NBP1-90242]

Staining of human cerebellum, endometrium, skeletal muscle and testis using NBP1-90242 (A) shows similar protein distribution across tissues to independent antibody NBP3-05523 (B).
ADAR Antibody Immunocytochemistry/ Immunofluorescence: ADAR Antibody [NBP1-90242]

Immunocytochemistry/ Immunofluorescence: ADAR Antibody [NBP1-90242]

Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli.

Applications for ADAR Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAR

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Double-stranded RNA-specific adenosine deaminase

Alternate Names

ADAR1, DRADA, DSRAD, EC 3.5.4.37, G1P1, IFI-4, IFI4, K88DSRBP, p136

Entrez Gene IDs

103 (Human); 56417 (Mouse); 81635 (Rat)

Gene Symbol

ADAR

UniProt

P55265 (Human)

Additional ADAR Products

Product Documents for ADAR Antibody

Certificate of Analysis

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Product Specific Notices for ADAR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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