Adenosine A3 R Antibody - BSA Free
Novus Biologicals | Catalog # NLS689
![Western Blot: Adenosine A3 R Antibody [NLS689]](https://resources.rndsystems.com/images/products/Adenosine-A3-R-Antibody-Western-Blot-NLS689-img0004.jpg "Western Blot: Adenosine A3 R Antibody [NLS689]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Porcine, Canine, Equine, Monkey, Primate
Cited:
Human, Mouse
Predicted:
Opossum (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Adenosine A3 R.
Epitope
3rd cytoplasmic domain
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30641086). Predicted cross-reactivity based on sequence identity: Marmoset (100%).
Specificity
Human ADORA3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for Adenosine A3 R Antibody - BSA Free
![Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689]](https://resources.rndsystems.com/images/products/Adenosine-A3-R-Antibody-Immunohistochemistry-Paraffin-NLS689-img0003.jpg "Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689]")
Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689]
Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689] - Analysis of anti-Adenosine A3 Receptor / ADORA3 antibody with human tonsil, squamous epithelium at dilution 20-30 ug/ml.
Immunohistochemistry: Adenosine A3 R Antibody [NLS689] -
Immunohistochemistry: Adenosine A3 R Antibody [NLS689] - si-CD73 inhibits HNSCC tumor growth & suppress EMT in vivo(A) Tumor growth curve of siRNA-CD73 mice & control mice. Data represent the mean ± SEM. of eight mice in each group. *P < 0.05, **P < 0.01, ***P < 0.001 by the Student's t-test; (B) Dissected tumors were photographed. The tumor volume & weight were measured. ***P < 0.001 by the Student's t-test; (C) Representative images of immunohistochemical analysis of CD73, p-EGFR, p-Src, A3R, Slug, E-cad, N-cad & Vimentin in tumors, (Scale bars = 100 μm). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.11435), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Adenosine A3 R Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
20-30 ug/ml
Application Notes
Use in Flow reported in scientific literature (PMID:34536555). Use in IHC and WB reported in scientific literature (PMID: 27557512).. Use in ICC/IF reported in scientific literature (PMID: 30641086)...
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: Adenosine A3 R
Long Name
Adenosine A3 Receptor
Alternate Names
A3AR, AD026, Adenosine A3R, ADORA3
Entrez Gene IDs
140 (Human)
Gene Symbol
ADORA3
UniProt
Additional Adenosine A3 R Products
Product Documents for Adenosine A3 R Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Adenosine A3 R Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Adenosine A3 R Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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