ADK Antibody

Novus Biologicals | Catalog # NBP2-15291

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ADK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADK. The exact sequence is proprietary.

Reactivity Notes

Chicken (83%),

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADK Antibody

Western Blot: ADK Antibody [NBP2-15291]

Western Blot: ADK Antibody [NBP2-15291]

Western Blot: ADK Antibody [NBP2-15291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ADK Antibody [NBP2-15291]

Immunocytochemistry/ Immunofluorescence: ADK Antibody [NBP2-15291]

Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP2-15291] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291]

Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291]

Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - Mouse liver. ADK stained by Adenosine kinase antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
ADK Antibody

Western Blot: ADK Antibody [NBP2-15291] -

Western Blot: ADK Antibody [NBP2-15291] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ADK antibody (NBP2-15291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ADK Antibody

Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] -

Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
ADK stained by ADK antibody (NBP2-15291) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ADK Antibody

Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] -

Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - ADK antibody detects ADK protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
ADK stained by ADK antibody (NBP2-15291) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ADK Antibody

Western Blot: ADK Antibody [NBP2-15291] -

Western Blot: ADK Antibody [NBP2-15291] - Wild-type (WT) and Adenosine kinase knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ADK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-15291 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ADK

This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq]

Long Name

Adenosine Kinase

Alternate Names

AK

Gene Symbol

ADK

UniProt

P55263

Additional ADK Products

Product Documents for ADK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ADK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for ADK Antibody (1)

4 out of 5
1 Customer Rating
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4 Stars
100%
3 Stars
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2 Stars
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1 Stars
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  • Name: Chiafeng Kuo
    Application: Western Blot
    Sample Tested:
    Species: Other
    Verified Customer | Posted 06/13/2015

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Protocols

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FAQs

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