Aggrecan Antibody (6-B-4)
Novus Biologicals | Catalog # NB110-6524
![Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524]](https://resources.rndsystems.com/images/products/Aggrecan-Antibody-6-B-4-Immunohistochemistry-Paraffin-NB110-6524-img0001.jpg "Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA
Cited:
Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6-B-4
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Product Specifications
Immunogen
Synthetic peptide: EPEEPFTFAPEI.
Reactivity Notes
Cross-reacts with Human and Cow. Sheep data from customer review. Mouse reactivity reported in scientific literature (PMID: 31364704).
Marker
Chondrocyte Marker
Specificity
Recognises the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobular domain (IGD) of human and bovine aggrecan.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aggrecan Antibody (6-B-4)
Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524]
Immunohistochemistry-Paraffin: Aggrecan Antibody (6-B-4) [NB110-6524] - Aggrecan on sheep intervertebral disc. Image from verified customer review.Applications for Aggrecan Antibody (6-B-4)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100
Application Notes
This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 review rated 5 using NB110-6524 in the following applications:
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Preservative
0.002% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aggrecan
Additional Aggrecan Products
Product Documents for Aggrecan Antibody (6-B-4)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Aggrecan Antibody (6-B-4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Aggrecan Antibody (6-B-4)
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Customer Reviews for Aggrecan Antibody (6-B-4) (1)
5 out of 5
1 Customer Rating
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Customer Images
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Application: Immunohistochemistry-ParaffinSample Tested: Sheep intervertebral discSpecies: OtherVerified Customer | Posted 08/04/2014Aggrecan on sheep intervertebral disc
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
