Aggrecan Antibody (6L4T2)

Novus Biologicals | Catalog # NBP3-33191

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 6L4T2
View all Aggrecan Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 761-861 of human Aggrecan (P16112).

Sequence:
WPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEES

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

261 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aggrecan Antibody (6L4T2)

Aggrecan Antibody (6L4T2)

Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] -

Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] - Immunohistochemistry analysis of paraffin-embedded Mouse bone marrow using Aggrecan Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Aggrecan Antibody (6L4T2)

Western Blot: Aggrecan Antibody (6L4T2) [NBP3-33191] -

Western Blot: Aggrecan Antibody (6L4T2) [NBP3-33191] - Western blot analysis of lysates from Mouse lung, using Aggrecan Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Aggrecan Antibody (6L4T2)

Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] -

Immunohistochemistry: Aggrecan Antibody (6L4T2) [NBP3-33191] - Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using Aggrecan Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Applications for Aggrecan Antibody (6L4T2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aggrecan

This aggrecan degradation product usually remains within the tissue still complexed to hyaluronan and link protein. Its release from the cartilage usually signals that there has been extensive catabolism of aggrecan, which allows large complexes containing this metabolite to be released from the tissue.

Alternate Names

ACAN, AGC1, CSPG1, CSPGCP, MSK16, SEDK

Gene Symbol

ACAN

Additional Aggrecan Products

Product Documents for Aggrecan Antibody (6L4T2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Aggrecan Antibody (6L4T2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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