Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human, Mouse, Rat, Rabbit

Predicted:

Sheep (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

AGTR2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human AGTR2. The immunogen is located within amino acids 220 - 270 of AGTR2. Amino Acid Squence: LKTNSYGKNRITRDQV

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:35372645).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AGTR-2 Antibody - BSA Free

Western Blot: AGTR-2 AntibodyBSA Free [NBP1-77368]

Western Blot: AGTR-2 AntibodyBSA Free [NBP1-77368]

Western Blot: AGTR-2 Antibody [NBP1-77368] - Mouse liver tissue lysate with Angiotensin II Type 2 Receptor antibody at 0.5 ug/ml.
Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368]

Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368]

AGTR-2-Antibody-Immunohistochemistry-NBP1-77368-img0009.jpg
Immunohistochemistry-Paraffin: AGTR-2 Antibody - BSA Free [NBP1-77368]

Immunohistochemistry-Paraffin: AGTR-2 Antibody - BSA Free [NBP1-77368]

AGTR-2-Antibody-Immunohistochemistry-Paraffin-NBP1-77368-img0010.jpg
AGTR-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of SOX2 [(A), red], PRR [(B), red], & ATIIR2 [(C), red] on GFAP+ CSCs [(A–C), green] & expression of ACE [(D), green] & ATIIR1 [(E), green] on SOX2+ CSCs [(D,E), red]. Negative control was a GBM tissue section with omission of the primary antibody (F). Cell nuclei were counterstained with 4′, 6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27730123), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
AGTR-2 Antibody - BSA Free

Western Blot: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Western Blot: AGTR-2 Antibody - BSA Free [NBP1-77368] - Effects of Ang(1-7) and/or A-779 treatments to the sham & OVX animals for 6 weeks on the expressions of RAS different proteins & osteoclastogenesis modulating factors in the femoral bone heads. (A) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL & OPG. (B) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns & bars represent the mean ± SEM of each group. Statistical significance was considered when *P < 0.05 & **P < 0.01 as compared to Sham group & #P < 0.05 & ##P < 0.01 as compared to OVX group. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-02570-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
AGTR-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
AGTR-2 Antibody - BSA Free

Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative 3,3-diaminobenzidine immunohistochemical-stained sections of moderately differentiated lip cancer samples showing cytoplasmic expression of (pro)renin receptor [(A), brown] within the tumor nests (TNs) & cells within the stroma. Angiotensin-converting enzyme [(B), brown] was expressed on the endothelium of the microvessels within the stroma but not the TNs. Angiotensin II receptor 1 [(C), brown] showed weak to moderate cytoplasmic & nuclear staining by cells within the TNs & cells within the stroma. Strong perinuclear & cytoplasmic staining for angiotensin II receptor 2 [(D), brown] was present in cells within the TNs & the stroma, & skeletal tissue. Nuclei were counterstained with hematoxylin [(A–D), blue]. Original magnification: 200×. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
AGTR-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
AGTR-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated lip squamous cell carcinoma demonstrating expression of (pro)renin receptor (PRR) [(A,B), red] by cells within the tumor nests (TNs) & the cells within the stroma that expressed SALL4 [(A), green]. PRR was also expressed by cells within the stroma that expressed OCT4 [(B), green]. Angiotensin-converting enzyme (ACE) [(C), green] was expressed on the endothelium of the microvessels which expressed SOX2 [(C), red], within the stroma. Staining with ETS-related gene [(G), red] confirmed that ACE [(G), green] was expressed by the endothelium of the microvessels within the stroma. Angiotensin II receptor 1 [(D), green] was demonstrated in cells within the TNs & occasional microvessels that stained positively for SOX2 [(D), red]. Angiotensin II receptor 2 [(E,F), red] was expressed by cells within the TNs, & cells within the stroma that expressed SALL4 [(E), green] & those that expressed OCT4 [(F), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
AGTR-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunocytochemistry of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 1 u/ml.
AGTR-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunofluorescence of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 5 ug/ml.
AGTR-2 Antibody - BSA Free

Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] -

Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunohistochemistry of AGTR-2 in rat liver tissue with AGTR-2 antibody at 5 ug/mL.

Applications for AGTR-2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID 27200356).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AGTR-2

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion that acts through at least two types of receptors, AGTR1 and AGTR2. It is an important effector controlling blood pressure and volume in the cardiovascular system and plays a major role in the development of the mammalian kidney and urinary tract. Like AGTR1, AGTR2 is a seven transmembrane G protein-coupled receptor (GPCR), but AGTR2 does not demonstrate most of the classic features of GPCR signaling. AGTR2 is involved in a wide range of activities, including the induction of neurite outgrowth and the inhibition of cellular proliferation, in addition to the known function of mediation of vasoconstriction.

Long Name

Angiostensin II Type 2 Receptor

Alternate Names

AGTR2, AT2, AT2R, MRX88

Gene Symbol

AGTR2

Additional AGTR-2 Products

Product Documents for AGTR-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AGTR-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AGTR-2 Antibody - BSA Free

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Protocols

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