AGTR-2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77368
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Rabbit
Cited:
Human, Mouse, Rat, Rabbit
Predicted:
Sheep (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
AGTR2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human AGTR2. The immunogen is located within amino acids 220 - 270 of AGTR2. Amino Acid Squence: LKTNSYGKNRITRDQV
Reactivity Notes
Use in Rabbit reported in scientific literature (PMID:35372645).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AGTR-2 Antibody - BSA Free
Western Blot: AGTR-2 AntibodyBSA Free [NBP1-77368]
Western Blot: AGTR-2 Antibody [NBP1-77368] - Mouse liver tissue lysate with Angiotensin II Type 2 Receptor antibody at 0.5 ug/ml.Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368]
AGTR-2-Antibody-Immunohistochemistry-NBP1-77368-img0009.jpgImmunohistochemistry-Paraffin: AGTR-2 Antibody - BSA Free [NBP1-77368]
AGTR-2-Antibody-Immunohistochemistry-Paraffin-NBP1-77368-img0010.jpgImmunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical stained images demonstrating the expression of SOX2 [(A), red], PRR [(B), red], & ATIIR2 [(C), red] on GFAP+ CSCs [(A–C), green] & expression of ACE [(D), green] & ATIIR1 [(E), green] on SOX2+ CSCs [(D,E), red]. Negative control was a GBM tissue section with omission of the primary antibody (F). Cell nuclei were counterstained with 4′, 6′-diamidino-2-phenylindole [(A–F), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27730123), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Western Blot: AGTR-2 Antibody - BSA Free [NBP1-77368] - Effects of Ang(1-7) and/or A-779 treatments to the sham & OVX animals for 6 weeks on the expressions of RAS different proteins & osteoclastogenesis modulating factors in the femoral bone heads. (A) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL & OPG. (B) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns & bars represent the mean ± SEM of each group. Statistical significance was considered when *P < 0.05 & **P < 0.01 as compared to Sham group & #P < 0.05 & ##P < 0.01 as compared to OVX group. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-02570-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative 3,3-diaminobenzidine immunohistochemical-stained sections of moderately differentiated lip cancer samples showing cytoplasmic expression of (pro)renin receptor [(A), brown] within the tumor nests (TNs) & cells within the stroma. Angiotensin-converting enzyme [(B), brown] was expressed on the endothelium of the microvessels within the stroma but not the TNs. Angiotensin II receptor 1 [(C), brown] showed weak to moderate cytoplasmic & nuclear staining by cells within the TNs & cells within the stroma. Strong perinuclear & cytoplasmic staining for angiotensin II receptor 2 [(D), brown] was present in cells within the TNs & the stroma, & skeletal tissue. Nuclei were counterstained with hematoxylin [(A–D), blue]. Original magnification: 200×. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated buccal mucosal squamous cell carcinoma demonstrating PRR [(A), red] was expressed by cells within the tumor nests that stained positively for EMA [(A), green]. PRR [(B), red] was also expressed by the CD34+ [(B), green] endothelium of the microvessels. In addition, RRR [(C), red] was expressed by cells within the peri-tumoral stroma, which also expressed OCT4 [(C), green]. ACE [(D), green] was expressed on the endothelium of the microvessels, which also expressed SOX2 [(D), red], within the peri-tumoral stroma. Cytoplasmic expression of ATIIR1 [(E), green] & PRR [(E), red] was demonstrated in cells within the tumor nests, the peri-tumoral stroma, & the endothelium of the microvessels within the peri-tumoral stroma. ATIIR2 [(F,G), red] was expressed by cells within the tumor nests that expressed SALL4 [(F), green] & OCT4 [(G), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27730124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Representative immunofluorescent immunohistochemical-stained sections of moderately differentiated lip squamous cell carcinoma demonstrating expression of (pro)renin receptor (PRR) [(A,B), red] by cells within the tumor nests (TNs) & the cells within the stroma that expressed SALL4 [(A), green]. PRR was also expressed by cells within the stroma that expressed OCT4 [(B), green]. Angiotensin-converting enzyme (ACE) [(C), green] was expressed on the endothelium of the microvessels which expressed SOX2 [(C), red], within the stroma. Staining with ETS-related gene [(G), red] confirmed that ACE [(G), green] was expressed by the endothelium of the microvessels within the stroma. Angiotensin II receptor 1 [(D), green] was demonstrated in cells within the TNs & occasional microvessels that stained positively for SOX2 [(D), red]. Angiotensin II receptor 2 [(E,F), red] was expressed by cells within the TNs, & cells within the stroma that expressed SALL4 [(E), green] & those that expressed OCT4 [(F), green]. Cell nuclei were counterstained with 4′,6′-diamidino-2-phenylindole [(A–G), blue]. Scale bars: 20 µm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunocytochemistry of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 1 u/ml.Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Immunocytochemistry/ Immunofluorescence: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunofluorescence of AGTR-2 in Jurkat cell with AGTR-2 Antibody at 5 ug/ml.Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] -
Immunohistochemistry: AGTR-2 Antibody - BSA Free [NBP1-77368] - Immunohistochemistry of AGTR-2 in rat liver tissue with AGTR-2 antibody at 5 ug/mL.Applications for AGTR-2 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1 ug/mL
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
0.5 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID 27200356).
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AGTR-2
Long Name
Angiostensin II Type 2 Receptor
Alternate Names
AGTR2, AT2, AT2R, MRX88
Gene Symbol
AGTR2
UniProt
Additional AGTR-2 Products
Product Documents for AGTR-2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AGTR-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for AGTR-2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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