AHR Antibody (3B12) - Azide and BSA Free

Novus Biologicals | Catalog # H00000196-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Cited:

Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B12

Format

Azide and BSA Free
View all AHR Primary Antibodies »

Product Specifications

Immunogen

AHR (NP_001612, 721 a.a. ~ 820 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINN

Specificity

aryl hydrocarbon receptor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AHR Antibody (3B12) - Azide and BSA Free

Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: AHR Antibody (3B12) [H00000196-M02]

Immunocytochemistry/ Immunofluorescence: AHR Antibody (3B12) [H00000196-M02]

Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).
Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02]

Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02]

Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02]

Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHR MaxPab rabbit polyclonal antibody.
ELISA: AHR Antibody (3B12) [H00000196-M02]

ELISA: AHR Antibody (3B12) [H00000196-M02]

ELISA: AHR Antibody (3B12) [H00000196-M02] - Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.

Applications for AHR Antibody (3B12) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.03ng/ml

Immunocytochemistry/ Immunofluorescence

20ug/mL

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF, RNAi Validation, and ELISA. ChIP usage reported in scientific literature (PMID: 23714156).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AHR

This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons.

Long Name

Aryl Hydrocarbon Receptor

Alternate Names

BHLHE76

Entrez Gene IDs

196 (Human)

Gene Symbol

AHR

OMIM

600253 (Human)

UniProt

NP_001612 (Human)

Additional AHR Products

Product Documents for AHR Antibody (3B12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AHR Antibody (3B12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AHR Antibody (3B12) - Azide and BSA Free

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Protocols

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FAQs

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