AHSA1 Antibody (25F2.D9) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-12883
![Western Blot: AHSA1 Antibody (25F2.D9)Azide and BSA Free [NBP2-12883]](https://resources.rndsystems.com/images/products/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Western-Blot-NBP2-12883-img0010.jpg "Western Blot: AHSA1 Antibody (25F2.D9)Azide and BSA Free [NBP2-12883]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # 25F2.D9
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant Full Length Mouse Aha1 Protein
Localization
Cytoplasm, Endoplasmic Reticulum
Specificity
Detects approx 38 kDa. Can run up to 45 kDa on SDS-PAGE.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for AHSA1 Antibody (25F2.D9) - Azide and BSA Free
Western Blot: AHSA1 Antibody (25F2.D9)Azide and BSA Free [NBP2-12883]
Western Blot: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Western Blot analysis of Human Cell lysates showing detection of AHSA1 protein using Rat Anti-AHSA1 Monoclonal Antibody, Clone 25F2.D9 (NBP2-12883). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody (NBP2-12883) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]](https://resources.rndsystems.com/images/products/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-12883-img0007.jpg "Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]")
Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]
Immunocytochemistry/Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).![Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]](https://resources.rndsystems.com/images/products/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-12883-img0011.jpg "Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]")
Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]
Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (NBP2-12883). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (NBP2-12883) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.![Immunoprecipitation: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]](https://resources.rndsystems.com/images/products/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-12883-img0009.jpg "Immunoprecipitation: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]")
Immunoprecipitation: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]
Immunoprecipitation: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Immunoprecipitation analysis using Rat Anti-AHSA1 Monoclonal Antibody, Clone 25F2.D9. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody at 1:1000.Applications for AHSA1 Antibody (25F2.D9) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:1000
Western Blot
1:1000
Application Notes
Use 1 ug/mL of AHA1 antibody was sufficient for detection of Aha1 in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
20 mM Potassium Phosphate, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AHSA1
Alternate Names
activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast), C14orf3, chromosome 14 open reading frame 3, p38AHA1
Gene Symbol
AHSA1
Additional AHSA1 Products
Product Documents for AHSA1 Antibody (25F2.D9) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AHSA1 Antibody (25F2.D9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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