AIF-1/Iba1 Antibody

Novus Biologicals | Catalog # NBP2-16908

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Porcine (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all AIF-1/Iba1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human AIF-1/Iba1. The exact sequence is proprietary.

Reactivity Notes

Bovine (88%).

Marker

pan-Microglia Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AIF-1/Iba1 Antibody

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908]

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908]

Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI.
Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908]

Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908]

Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908]

Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908]

Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).
AIF-1/Iba1 Antibody

Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] -

Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-16908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
AIF-1/Iba1 Antibody

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] -

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
AIF-1/Iba1 Antibody

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] -

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
AIF-1/Iba1 Antibody

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] -

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
AIF-1/Iba1 Antibody

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] -

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
AIF-1/Iba1 Antibody

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] -

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (NBP2-16908) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
AIF-1/Iba1 Antibody

Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (NBP2-16908) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
AIF-1/Iba1 Antibody

Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] -

Miconazole effects on NO generation and inflammation in LPS-treated cultured astrocytes and microglial BV2 cells.The NO generation levels were measured in 24-h LPS-treated (1 μg/mL) cultured astrocytes (a) and microglial BV2 cells (b) pretreated with MCZ (1.25, 2.5, 5, and 10 μM) for 1 h. COX-2, GFAP, and Iba-1 proteins were detected by western blotting using specific antibodies in cultured astrocytes (c) and microglial BV2 cells (d). beta -actin levels were measured to confirm equal protein loading. All experiments were performed three times with duplicate. The values on the western blot bands represent the arbitrary density measured by ImageJ. Significant difference from the control group (t-test, p < 0.05). #Significant difference from the LPS-treated group (p < 0.05). *Significant difference from the MCZ-treated group (p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32796824), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for AIF-1/Iba1 Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AIF-1/Iba1

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T.,... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Long Name

Allograft Inflammatory Factor 1

Alternate Names

AIF1, IBA1, IRT1

Gene Symbol

AIF1

Additional AIF-1/Iba1 Products

Product Documents for AIF-1/Iba1 Antibody

Certificate of Analysis

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Product Specific Notices for AIF-1/Iba1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AIF-1/Iba1 Antibody

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Protocols

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