AIF-1/Iba1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-19019

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Primate - Macaca mulatta (Rhesus Macaque)

Predicted:

Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all AIF-1/Iba1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AIF-1/Iba1. The exact sequence is proprietary.

Marker

pan-Microglia Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit AIF-1/Iba1 Antibody - Azide and BSA Free (NBP2-19019) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF and Simple Western. Anti-AIF-1/Iba1 Antibody: Cited in 29 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for AIF-1/Iba1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse fore brain. Iba1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019]

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019]

Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 Antibody (1 1:1000, Red) expression in dorsal root ganglia. Green fluorescent Nissl was used as counter-staining. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse cerebellum stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat brain stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat cerebellum stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat hind brain. Iba1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Analysis of paraffin-embedded human hepatoma, using at 1:500 dilution.Iba1 antibody detects AIF-1/Iba1 in the cytosol of Kupffer cell in human hepatoma by immunohistochemical analysis.
AIF-1/Iba1 Antibody

Immunohistochemistry: AIF-1/Iba1 Antibody [NBP2-19019] -

nbp2-19019_rabbit-polyclonal-aif-1-iba1-antibody-2552023153956.jpg
AIF-1/Iba1 Antibody

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - LCN2 mainly expressed on neurons. a Representative immunofluorescence images of LCN2 (green), GFAP (red), NeuN (red) & IbaI (red) staining in sections of the hippocampus after surgery. Long scale bar, 600 µm; short scale bar, 25 µm. b Percentage quantification of LCN2 positive cells (n = 3 mice/group). c Cartoon depicting MACS sorting procedures for isolating CD11b+ & CD11b − cells. d Relative expression of cx3cr1 in CD11b + & CD11b − cells after sorting from all samples. d Relative expression level of Lcn2 mRNA in CD11b + & CD11b − cells from hippocampus 6 h after surgery. e Relative expression level of IL-6 mRNA in CD11b + cells. Data are represented as mean ± SEM. **** P < 0.0001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35413913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
AIF-1/Iba1 Antibody

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - Knockdown of LCN2 prevents microglial activation & neuroinflammation. a Representative immunofluorescence images of hippocampal sections with IbaI+ cells (White). Scale bars, 150 µm. b Quantification of the microglia density in (A) (n = 4 mice/group, 2–3 slides/mice). c Quantification of the microglia Ramification index at 24 h after surgery (n = 72 cells from 4 mice/group). d Number of process intersections with shells at distances (in 2 µm in increments) from the soma by Sholl analysis at 24 h after surgery (n = 72 cells from 4 mice/group). e Tissue ELISA of IL-6 at 24 h after surgery (n = 5 mice/group). f Relative expression level of tnf-alpha, IL-6 and/or IL-1 beta 6 h after application of rLCN2 (500 ng/mL) on BV-2 (n = 4–6/group) & primary microglia cells (n = 3/group). Vehicle: PBS buffer. Data represent mean ± SEM; n = 4/group. * P < 0.05; *** P < 0.001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35413913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
AIF-1/Iba1 Antibody

Immunohistochemistry: AIF-1/Iba1 Antibody [NBP2-19019] -

Immunohistochemistry: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 antibody detects AIF-1/Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: ice-cold MeOH for 5 min
AIF-1/Iba1 Antibody

Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] -

Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:1000.
AIF-1/Iba1 Antibody

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] -

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 antibody detects AIF-1/Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
AIF-1/Iba1 stained by AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
AIF-1/Iba1 Antibody

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] -

Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 antibody detects AIF-1/Iba1 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:500.
Blue: Fluoroshield with DAPI.
AIF-1/Iba1 Antibody

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] -

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 antibody detects AIF-1/Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AIF-1/Iba1 stained by AIF-1/Iba1 antibody (NBP2-19019) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
AIF-1/Iba1 Antibody - Azide and BSA Free

Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-19019] -

Iba1 antibody (NBP2-19019) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (NBP2-19019) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
AIF-1/Iba1 Antibody - Azide and BSA Free

Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NBP2-19019] -

Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Iba1 protein stained by Iba1 antibody (NBP2-19019) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
AIF-1/Iba1 Antibody - Azide and BSA Free

Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] -

Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (NBP2-19019) diluted at 1:1000.
AIF-1/Iba1 Antibody - Azide and BSA Free

Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (NBP2-19019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
AIF-1/Iba1 Antibody - Azide and BSA Free

Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] -

Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (NBP2-19019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for AIF-1/Iba1 Antibody - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1000

Immunohistochemistry Free-Floating

Assay dependent

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

1:50

Western Blot

1:500-1:10000
Application Notes
Simple Western Separated by Size, antibody dilution of 1:50, detects a band at 25 kDa.

Reviewed Applications

Read 4 reviews rated 3.8 using NBP2-19019 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AIF-1/Iba1

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T.,... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Long Name

Allograft Inflammatory Factor 1

Alternate Names

AIF1, IBA1, IRT1

Entrez Gene IDs

199 (Human)

Gene Symbol

AIF1

Additional AIF-1/Iba1 Products

Product Documents for AIF-1/Iba1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AIF-1/Iba1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AIF-1/Iba1 Antibody - Azide and BSA Free

Customer Reviews for AIF-1/Iba1 Antibody - Azide and BSA Free (4)

3.8 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
25%
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0%
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1 Stars
25%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • AIF-1/Iba1 Antibody
    Name: Casey Nestor
    Application: Immunohistochemistry-Frozen
    Sample Tested: Brain (hypothalamus) tissue
    Species: Sheep
    Verified Customer | Posted 03/13/2020
    Photomicrograph (40x magnification) of sheep hypothalamus stained using IBA-1 (NBP2-19019) at dilution of 1:100. No positive staining was detected.
    To test, we used free-floating sheep hypothalamic brain sections from two animals. We did dilutions of 1:100 to 1:1000 and did not see any staining in our tissue. We also employed an antigen retrieval step in one of our runs, and it did not yield any different results.
    AIF-1/Iba1 Antibody - Azide and BSA Free NBP2-19019
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • AIF-1/Iba1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse forebrain lysate
    Species: Mouse
    Verified Customer | Posted 12/18/2017
    Iba1 expression in mouse forebrain lysate under basal and activated conditions
    AIF-1/Iba1 Antibody - Azide and BSA Free NBP2-19019
  • AIF-1/Iba1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: C57BL/6 mouse retina
    Species: Mouse
    Verified Customer | Posted 04/01/2017
  • AIF-1/Iba1 Antibody
    Name: Temugin Berta
    Application: Immunocytochemistry
    Sample Tested: Dorsal root ganglia
    Species: Mouse
    Verified Customer | Posted 03/10/2017
    AIF-1/Iba1 (Red, NBP2-19019) expression in dorsal root ganglia. Green fluorescent Nissl was used as counter-staining.
    Blocking and antibody solution: BSA1%/Triton 02% in PBS Dilution AIF1: 1/1000 Secondary antibody: Alexa 555 Counter-staining: Nissl
    AIF-1/Iba1 Antibody - Azide and BSA Free NBP2-19019

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FAQs for AIF-1/Iba1 Antibody - Azide and BSA Free

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    • Q: For  prepared antibody NBP2-19019, what is the corresponding  amino acid sequence?
      A: The AIF-1/Iba1 antibody (NBP2-19019) was generated using a carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary, but we can share that it is within aa 97-147 (Isoform 1) - https://www.uniprot.org/uniprot/P55008).  The full-length human form for this one is 147aa total.Using the exact sequence, there is 100% identity to - https://www.uniprot.org/uniprot/Q5TM25
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