AIF Antibody (4E7)

Novus Biologicals | Catalog # NBP2-37577

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4E7
View all AIF Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human AIF expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse AIF Antibody (4E7) (NBP2-37577) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-AIF Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for AIF Antibody (4E7)

Western Blot: AIF Antibody (4E7)BSA Free [NBP2-37577]

Western Blot: AIF Antibody (4E7)BSA Free [NBP2-37577]

Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: AIF Antibody (4E7)BSA Free [NBP2-37577]

Immunocytochemistry/ Immunofluorescence: AIF Antibody (4E7)BSA Free [NBP2-37577]

Immunocytochemistry/Immunofluorescence: AIF Antibody (4E7) [NBP2-37577] - Analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Immunohistochemistry-Paraffin: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human lung cancer tissues using AIF mouse mAb with DAB staining.
Flow Cytometry: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Flow Cytometry: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Flow Cytometry: AIF Antibody (4E7) [NBP2-37577] - Analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).
Western Blot: AIF Antibody (4E7)BSA Free [NBP2-37577]

Western Blot: AIF Antibody (4E7)BSA Free [NBP2-37577]

Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)
Immunohistochemistry-Paraffin: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Immunohistochemistry-Paraffin: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human breast cancer tissues using AIF mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Immunohistochemistry-Paraffin: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human cervical cancer tissues using AIF mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Immunohistochemistry-Paraffin: AIF Antibody (4E7) - BSA Free [NBP2-37577]

Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human rectum cancer tissues using AIF mouse mAb with DAB staining.
ELISA: AIF Antibody (4E7) - BSA Free [NBP2-37577]

ELISA: AIF Antibody (4E7) - BSA Free [NBP2-37577]

ELISA: AIF Antibody (4E7) [NBP2-37577] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for AIF Antibody (4E7)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AIF

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Long Name

Apoptosis Inducing Factor

Alternate Names

AIFM1, COXPD6, PDCD8

Gene Symbol

AIFM1

Additional AIF Products

Product Documents for AIF Antibody (4E7)

Certificate of Analysis

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Product Specific Notices for AIF Antibody (4E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for AIF Antibody (4E7)

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail