AIF Antibody

Novus Biologicals | Catalog # NBP2-42496

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AIF Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NEVAKLFNIHED corresponding to C-Terminus according to NP_665811.1, NP_665812.1, NP_001124318.1.

Reactivity Notes

Expected from sequence similarity: Bovine

Specificity

This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat AIF Antibody (NBP2-42496) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-AIF Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for AIF Antibody

Western Blot: AIF Antibody [NBP2-42496]

Western Blot: AIF Antibody [NBP2-42496]

Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: AIF Antibody [NBP2-42496]

Immunocytochemistry/ Immunofluorescence: AIF Antibody [NBP2-42496]

Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).
Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42496]

Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42496]

Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42496] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: AIF Antibody [NBP2-42496]

Western Blot: AIF Antibody [NBP2-42496]

Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: AIF Antibody [NBP2-42496]

Western Blot: AIF Antibody [NBP2-42496]

Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A) and Rat (B) Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: AIF Antibody [NBP2-42496]

Western Blot: AIF Antibody [NBP2-42496]

Western Blot: AIF Antibody [NBP2-42496] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: AIF Antibody [NBP2-42496]

Immunocytochemistry/ Immunofluorescence: AIF Antibody [NBP2-42496]

Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).

Applications for AIF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 - 0.1 ug/mL
Application Notes
Western blot: Approx 70 kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65 kDa observed in Pig Heart lysates (calculated MW of 66.9 kDa according to Human NP_004199.1 and 66.8 kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1).
Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AIF

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Long Name

Apoptosis Inducing Factor

Alternate Names

AIFM1, COXPD6, PDCD8

Entrez Gene IDs

9131 (Human)

Gene Symbol

AIFM1

Additional AIF Products

Product Documents for AIF Antibody

Certificate of Analysis

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Product Specific Notices for AIF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail