AIF Antibody

Novus Biologicals | Catalog # NBP2-42509

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AIF Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DDPNVTKTLRFKQ corresponding to internal region according to NP_004199.1, NP_665811.1, NP_001124319.1.

Specificity

This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AIF Antibody

Western Blot: AIF Antibody [NBP2-42509]

Western Blot: AIF Antibody [NBP2-42509]

Western Blot: AIF Antibody [NBP2-42509] - Staining of Jurkat lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: AIF Antibody [NBP2-42509]

Immunocytochemistry/ Immunofluorescence: AIF Antibody [NBP2-42509]

Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42509] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509]

Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509]

Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunocytochemistry/ Immunofluorescence: AIF Antibody [NBP2-42509]

Immunocytochemistry/ Immunofluorescence: AIF Antibody [NBP2-42509]

Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42509] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509]

Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509]

Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509] - Staining of paraffin embedded Human Kidney with antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for AIF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/mL

Immunohistochemistry

5 - 7 ug/mL

Immunohistochemistry-Paraffin

5 - 7 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AIF

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Long Name

Apoptosis Inducing Factor

Alternate Names

AIFM1, COXPD6, PDCD8

Entrez Gene IDs

9131 (Human)

Gene Symbol

AIFM1

Additional AIF Products

Product Documents for AIF Antibody

Certificate of Analysis

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Product Specific Notices for AIF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail