AKAP12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89168

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AKAP12 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QRAEAERPEEQAEASGLKKETDVVLKVDAQEAKTEPFTQGKVVGQTTPESFEKAPQVTESIESSELVTTCQAETLAGVKSQEMVMEQAIPPDSVETPTDSETDGST

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKAP12 Antibody - BSA Free

Western Blot: AKAP12 Antibody [NBP1-89168]

Western Blot: AKAP12 Antibody [NBP1-89168]

Western Blot: AKAP12 Antibody [NBP1-89168] - Analysis in human cell lines U-251MG and MCF-7 using anti-AKAP12 antibody. Corresponding AKAP12 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: AKAP12 Antibody [NBP1-89168]

Immunocytochemistry/ Immunofluorescence: AKAP12 Antibody [NBP1-89168]

Immunocytochemistry/Immunofluorescence: AKAP12 Antibody [NBP1-89168] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining in human testis and skeletal muscle tissues using NBP1-89168 antibody. Corresponding AKAP12 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human endometrium shows moderate positivity in cells in endometrial stroma.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human endometrium, kidney, skeletal muscle and testis using Anti-AKAP12 antibody NBP1-89168 (A) shows similar protein distribution across tissues to independent antibody NBP2-38786 (B).
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human kidney shows strong positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-89168] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Applications for AKAP12 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF fixation permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKAP12

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

A kinase (PRKA) anchor protein (gravin) 12, A kinase (PRKA) anchor protein 12, AKAP 250, AKAP250250kDa, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621, Gravin, kinase scaffold protein gravin, Myasthenia gravis autoantigen, myasthenia gravis autoantigen gravin

Entrez Gene IDs

9590 (Human)

Gene Symbol

AKAP12

UniProt

Q02952 (Human)

Additional AKAP12 Products

Product Documents for AKAP12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for AKAP12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AKAP12 Antibody - BSA Free

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Protocols

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FAQs

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