Albumin Antibody (1G12-1B3) - Azide and BSA Free

Novus Biologicals | Catalog # H00000213-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G12-1B3

Format

Azide and BSA Free
View all Albumin Primary Antibodies »

Product Specifications

Immunogen

ALB (AAH34023, 19 a.a. ~ 609 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEGGKKLVAASQAALGL

Specificity

ALB - albumin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Albumin Antibody (1G12-1B3) - Azide and BSA Free

Western Blot: Albumin Antibody (1G12-1B3) [H00000213-M01]

Western Blot: Albumin Antibody (1G12-1B3) [H00000213-M01]

Western Blot: Albumin Antibody (1G12-1B3) [H00000213-M01] - Analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody (M01), clone 1G12-1B3.Lane 1: ALB transfected lysate(69.4 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Albumin Antibody (1G12-1B3) [H00000213-M01]

Immunohistochemistry-Paraffin: Albumin Antibody (1G12-1B3) [H00000213-M01]

Immunohistochemistry-Paraffin: Albumin Antibody (1G12-1B3) [H00000213-M01] - Analysis of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. Antibody concentration 3 ug/ml.
Western Blot: Albumin Antibody (1G12-1B3) [H00000213-M01]

Western Blot: Albumin Antibody (1G12-1B3) [H00000213-M01]

Western Blot: Albumin Antibody (1G12-1B3) [H00000213-M01] - ALB expression in HepG2 ( Cat # L019V1 ).
Immunoprecipitation: Albumin Antibody (1G12-1B3) [H00000213-M01]

Immunoprecipitation: Albumin Antibody (1G12-1B3) [H00000213-M01]

Immunoprecipitation: Albumin Antibody (1G12-1B3) [H00000213-M01] - Analysis of ALB transfected lysate using anti-ALB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALB MaxPab rabbit polyclonal antibody.
ELISA: Albumin Antibody (1G12-1B3) [H00000213-M01]

ELISA: Albumin Antibody (1G12-1B3) [H00000213-M01]

ELISA: Albumin Antibody (1G12-1B3) [H00000213-M01] - Detection limit for recombinant GST tagged ALB is approximately 1ng/ml as a capture antibody.

Applications for Albumin Antibody (1G12-1B3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Albumin

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Alternate Names

ALB

Entrez Gene IDs

213 (Human)

Gene Symbol

ALB

OMIM

103600 (Human)

UniProt

AAH34023 (Human)

Additional Albumin Products

Product Documents for Albumin Antibody (1G12-1B3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Albumin Antibody (1G12-1B3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Phase Proteins

Citations for Albumin Antibody (1G12-1B3) - Azide and BSA Free

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Protocols

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FAQs

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