alcohol dehydrogenase 4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90233

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alcohol dehydrogenase 4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALGATDCLNPRDLHKPIQEVIIELTKGGVDFALDCAGGSETMKAALDCTTAGWGSCTFIGVAAGSKGLTVFPEELIIGRTINGTFFGGWKSVDSIPKLVTDYKNKKFNLDALV

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24309898)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alcohol dehydrogenase 4 Antibody - BSA Free

alcohol dehydrogenase 4 Antibody - BSA Free Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Analysis in human liver and tonsil tissues using NBP1-90233 antibody. Corresponding ADH4 RNA-seq data are presented for the same tissues.
alcohol dehydrogenase 4 Antibody - BSA Free Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
alcohol dehydrogenase 4 Antibody - BSA Free Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Staining of human tonsil shows no positivity in germinal center cells as expected.
alcohol dehydrogenase 4 Antibody - BSA Free Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Staining of human fallopian tube shows no positivity in glandular cells as expected.
alcohol dehydrogenase 4 Antibody - BSA Free Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Immunohistochemistry: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Staining of human duodenum shows strong cytoplasmic positivity in endocrine glandular cells.
alcohol dehydrogenase 4 Antibody - BSA Free Western Blot: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Western Blot: alcohol dehydrogenase 4 Antibody - BSA Free [NBP1-90233]

Analysis in human liver tissue.

Applications for alcohol dehydrogenase 4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 4

alcohol dehydrogenase 4 encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Alternate Names

ADH-2, alcohol dehydrogenase 4, alcohol dehydrogenase 4 (class II), pi polypeptide, Alcohol dehydrogenase class II pi chain, aldehyde reductase, EC 1.1.1, EC 1.1.1.1

Entrez Gene IDs

127 (Human)

Gene Symbol

ADH4

UniProt

P08319 (Human)

Additional alcohol dehydrogenase 4 Products

Product Documents for alcohol dehydrogenase 4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for alcohol dehydrogenase 4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alcohol dehydrogenase 4 Antibody - BSA Free

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Protocols

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