alcohol dehydrogenase 5 Antibody (3E7) - BSA Free

Novus Biologicals | Catalog # NBP3-42978

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3E7

Format

BSA Free
View all alcohol dehydrogenase 5 Primary Antibodies »

Product Specifications

Immunogen

alcohol dehydrogenase 5(class III), chi polypeptide - (Uniprot# P11766)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse alcohol dehydrogenase 5 Antibody (3E7) - BSA Free (NBP3-42978) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for alcohol dehydrogenase 5 Antibody (3E7) - BSA Free

alcohol dehydrogenase 5 Antibody (3E7)

Western Blot: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] -

Western Blot: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] - fetal human brain tissue were subjected to SDS PAGE followed by western blot with (alcohol dehydrogenase 5 Antibody) at dilution of 1:4000
alcohol dehydrogenase 5 Antibody (3E7)

Immunohistochemistry: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] -

Immunohistochemistry: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] - Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using (alcohol dehydrogenase 5 Antibody) at dilution of 1:50
alcohol dehydrogenase 5 Antibody (3E7)

Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] -

Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody (3E7) [NBP3-42978] - Immunofluorescent analysis of HepG2 cells using (alcohol dehydrogenase 5 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG

Applications for alcohol dehydrogenase 5 Antibody (3E7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Immunohistochemistry

1:20 - 1:200

Western Blot

1:1000 - 1:8000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 5

The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.

Alternate Names

ADH-3, ADHXEC 1.1.1.1, alcohol dehydrogenase (class III), chi polypeptide, Alcohol dehydrogenase 5, alcohol dehydrogenase 5 (class III), chi polypeptide, Alcohol dehydrogenase class chi chain, alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, EC 1.1.1, EC 1.1.1.-, EC 1.1.1.284, FALDH, FDHGSNOR, formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, S-(hydroxymethyl)glutathione dehydrogenase

Gene Symbol

ADH5

Additional alcohol dehydrogenase 5 Products

Product Documents for alcohol dehydrogenase 5 Antibody (3E7) - BSA Free

Certificate of Analysis

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Product Specific Notices for alcohol dehydrogenase 5 Antibody (3E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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