ALDH18A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83324

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALDH18A1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ALDH18A1 Antibody - BSA Free

Western Blot: ALDH18A1 Antibody [NBP1-83324]

Western Blot: ALDH18A1 Antibody [NBP1-83324]

Western Blot: ALDH18A1 Antibody [NBP1-83324] - Analysis using Anti-ALDH18A1 antibody NBP1-83324 (A) shows similar pattern to independent antibody NBP1-83325 (B).
Immunocytochemistry/ Immunofluorescence: ALDH18A1 Antibody [NBP1-83324]

Immunocytochemistry/ Immunofluorescence: ALDH18A1 Antibody [NBP1-83324]

Immunocytochemistry/Immunofluorescence: ALDH18A1 Antibody [NBP1-83324] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324]

Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324]

Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324] - Staining of human gastrointestinal shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324]

Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324]

Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324]

Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324]

Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324]

Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324]

Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324] - Staining of human liver shows no positivity in hepatocytes as expected.
ALDH18A1 Antibody - BSA Free Western Blot: ALDH18A1 Antibody - BSA Free [NBP1-83324]

Western Blot: ALDH18A1 Antibody - BSA Free [NBP1-83324]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
ALDH18A1 Antibody - BSA Free Western Blot: ALDH18A1 Antibody - BSA Free [NBP1-83324]

Western Blot: ALDH18A1 Antibody - BSA Free [NBP1-83324]

Analysis in human cell line HepG2.

Applications for ALDH18A1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH18A1

ALDH18A1, or Delta-1-pyrroline-5-carboxylate synthase, contains a long 88 kDa and a short 87 kDa isoform, and is involved in the catalytic conversion of glutamate to glutamate 5-semialdehyde. Current research on ALDH18A1 is being conducted to determine the relation between the protein and a variety of diseases and disorders, including neurodegeneration, pneumonia, connective tissue disease, mental retardation, hypoornithinemia, tuberculosis, microcephaly, Alzheimer's disease, gastroesophageal reflux disease, and dementia. The protein has been linked to biosynthesis and metabolic pathways where it interacts with PUS1, UQCRFS1, ICT1, OAT, and MYC.

Alternate Names

aldehyde dehydrogenase 18 family, member A1, Aldehyde dehydrogenase family 18 member A1, delta-1-pyrroline-5-carboxylate synthase, EC 2.1.2.11, EC 2.3.3.13, GSASdelta1-pyrroline-5-carboxlate synthetase, MGC117316, P5CSdelta-1-pyrroline-5-carboxylate synthetase, PYCSEC 1.2.1.41, pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase)

Entrez Gene IDs

5832 (Human)

Gene Symbol

ALDH18A1

UniProt

P54886 (Human)

Additional ALDH18A1 Products

Product Documents for ALDH18A1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ALDH18A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ALDH18A1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ALDH18A1 Antibody - BSA Free

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  • Q: I am looking for mouse Aldh18a1 antibody suitable for immunohistochemistry, and find your antibodies NBP1-83324 and NBP1-83325. Do you have the data of WB or IHC using these antibodies against mouse samples? If not, I would like to test them in our samples. It would be great if you could give us an aliquot of them, and we will let you know the outcomes.

    A:

    For our two products NBP1-83324 and NBP1-83325, we have not yet tested these products for detection of the mouse protein. I did run alignments of our antigens against the mouse sequence. Both sequences have a high homology to the mouse sequence. Since we have not yet tested these against mouse samples, you would qualify for our Innovator's Reward program. In exchange for a review of these products in your experiment, we would issue you a credit for the purchase price of the antibody. Regardless of results. Due to our Innovator's Reward Program, we do not offer free aliquots of our products. I am sorry for any inconvenience.

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