ALDH1A2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87158

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALDH1A2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VVGSPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFGPVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTV

Marker

Dorso-temporal Retina Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1A2 Antibody - BSA Free

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining in human endometrium and skin tissues. Corresponding ALDH1A2 RNA-seq data are presented for the same tissues.
Western Blot: ALDH1A2 Antibody [NBP1-87158]

Western Blot: ALDH1A2 Antibody [NBP1-87158]

Western Blot: ALDH1A2 Antibody [NBP1-87158] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in cells in endometrial stroma.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human skin shows weak cytoplasmic positivity in a subset of keratinocytes.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Simple Western: ALDH1A2 Antibody [NBP1-87158]

Simple Western: ALDH1A2 Antibody [NBP1-87158]

Simple Western: ALDH1A2 Antibody [NBP1-87158] - Detection of ALDH1A2 in K562 cells using ALDH1A2 antibody at a dilution of 1:50. K (lane 1) = Wild Type K562, I (lane 2) = Imatinib Resistant K562, N (lane 3) = Nilotinib Resistant K562 and D (lane 4) = Dasatinib Resistant K562. Image submitted by a verified customer review.
ALDH1A2 Antibody

Immunohistochemistry: ALDH1A2 Antibody [NBP1-87158] -

Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, & TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ALDH1A2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
See Simple Western Antibody Database for Simple Western validation: Tested in Wild Type K562 cells, Imatinib Resistant K562 cells, Nilotinib Resistant K562 cells and Dasatinib Resistant K56 cells, separated by Size, antibody dilution of 1:50

Reviewed Applications

Read 1 review rated 5 using NBP1-87158 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH1A2

ALDH1A2 belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Aldehyde Dehydrogenase 1 Family, Member A2

Alternate Names

ALDH 1A2, RALDH, RALDH2

Entrez Gene IDs

8854 (Human)

Gene Symbol

ALDH1A2

UniProt

O94788 (Human)

Additional ALDH1A2 Products

Product Documents for ALDH1A2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ALDH1A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for ALDH1A2 Antibody - BSA Free

Customer Reviews for ALDH1A2 Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Simple Western
    Sample Tested: k562 cells
    Species: Human
    Verified Customer | Posted 08/18/2016
    Simple Western analysis of untreated or treated K562 cells using ALDH1A2 antibody
    ALDH1A2 Antibody - BSA Free NBP1-87158

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Protocols

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FAQs for ALDH1A2 Antibody - BSA Free

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  • Q: I am looking for an anti-human ALDH1A2 antibody to use for flow cytometry and I’m wondering if anyone has used NBP1-87158 for this purpose?

    A: Unfortunately we have not yet investigated the use of product NBP1-87158 in flow cytometry yet. This antibody is validated for use in IHC and ICC, so it is very likely that it would work in flow as well. Since we are not able to cover this application with our guarantee, I can offer you our Innovator's Reward Program.

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