ALDH1A2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92915

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALDH1A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALDH1A2 (NP_733797.1). MTSSKIEMPGEVKADPAALMASLHLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVYNPATGEQVCEVQEADKADIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRAVLATMESLNGGKPFLQAFYVDLQGVIKTFRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1A2 Antibody - BSA Free

Western Blot: ALDH1A2 AntibodyBSA Free [NBP2-92915]

Western Blot: ALDH1A2 AntibodyBSA Free [NBP2-92915]

Western Blot: ALDH1A2 Antibody [NBP2-92915] - Analysis of extracts of various cell lines, using ALDH1A2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: ALDH1A2 Antibody - BSA Free [NBP2-92915]

Immunocytochemistry/ Immunofluorescence: ALDH1A2 Antibody - BSA Free [NBP2-92915]

Immunocytochemistry/Immunofluorescence: ALDH1A2 Antibody [NBP2-92915] - Analysis of NIH-3T3 cells using ALDH1A2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody - BSA Free [NBP2-92915]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody - BSA Free [NBP2-92915]

Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP2-92915] - Rat kidney using ALDH1A2 antibody at dilution of 1:100 (40x lens).
Immunocytochemistry/ Immunofluorescence: ALDH1A2 Antibody - BSA Free [NBP2-92915]

Immunocytochemistry/ Immunofluorescence: ALDH1A2 Antibody - BSA Free [NBP2-92915]

Immunocytochemistry/Immunofluorescence: ALDH1A2 Antibody [NBP2-92915] - Analysis of C6 cells using ALDH1A2 at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for ALDH1A2 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALDH1A2

ALDH1A2 belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Aldehyde Dehydrogenase 1 Family, Member A2

Alternate Names

ALDH 1A2, RALDH, RALDH2

Gene Symbol

ALDH1A2

Additional ALDH1A2 Products

Product Documents for ALDH1A2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ALDH1A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for ALDH1A2 Antibody - BSA Free

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Protocols

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