ALG5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88767

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ALG5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RHEEEKFFLNAKGQKETLPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQTSKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKLEKGLNDL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALG5 Antibody - BSA Free

Western Blot: ALG5 Antibody [NBP1-88767]

Western Blot: ALG5 Antibody [NBP1-88767]

Western Blot: ALG5 Antibody [NBP1-88767] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: ALG5 Antibody [NBP1-88767]

Immunohistochemistry-Paraffin: ALG5 Antibody [NBP1-88767]

Immunohistochemistry-Paraffin: ALG5 Antibody [NBP1-88767] - Staining of human rectum shows cytoplasmic positivity in glandular cells.
Western Blot: ALG5 Antibody [NBP1-88767]

Western Blot: ALG5 Antibody [NBP1-88767]

Western Blot: ALG5 Antibody [NBP1-88767] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
ALG5 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: ALG5 Antibody [NBP1-88767]

Immunocytochemistry/ Immunofluorescence: ALG5 Antibody [NBP1-88767]

Staining of human cell line U-251 MG shows localization to cytosol.

Applications for ALG5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALG5

ALG5, also known as Dolichyl-phosphate beta-glucosyltransferase, has a 37 kDa and a 34 kDa isoform, and is involved in the process of substrate recognition. Disease research is currently being conducted on ALG5 and its relation to the congenital disorder of glycosylation. The protein has been linked to several protein metabolism, biosynthesis, and modification pathways, where it interacts with ACER3, DDOST, PIGK, RPN1, and ALG12.

Alternate Names

Alg5, S. cerevisiae, homolog of, asparagine-linked glycosylation 5 homolog (S. cerevisiae, dolichyl-phosphatebeta-glucosyltransferase), asparagine-linked glycosylation 5 homolog (yeast, dolichyl-phosphatebeta-glucosyltransferase), asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferasehomolog (S. cerevisiae), Asparagine-linked glycosylation protein 5 homolog, bA421P11.2, dolichyl phosphate glucosyltransferase, dolichyl-phosphate beta-glucosyltransferase, dolP-glucosyltransferase, EC 2.4.1, EC 2.4.1.117, RP11-421P11.2

Entrez Gene IDs

29880 (Human)

Gene Symbol

ALG5

UniProt

Q9Y673 (Human)

Additional ALG5 Products

Product Documents for ALG5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ALG5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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