Alkaline Phosphatase/ALPP Antibody (JM22-53)
Novus Biologicals | Catalog # NBP2-67136
Recombinant Monoclonal Antibody
![Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Western-Blot-NBP2-67136-img0005.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JM22-53 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human Alkaline Phosphatase/ALPP aa 171-220 / 535. (SwissProt: P05187 Human)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (JM22-53)
Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]
Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of Placental alkaline phosphatase/ALPP on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase/ALPP antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunocytochemistry-Immunofluorescence-NBP2-67136-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]")
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Staining PLAP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0007.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]")
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human seminoma tissue with Rabbit anti-Placental alkaline phosphatase/ALPP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Western-Blot-NBP2-67136-img0004.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]")
Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]
Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunocytochemistry-Immunofluorescence-NBP2-67136-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]")
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Staining PLAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0003.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]")
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human placenta tissue using anti-PLAP antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0006.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]")
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136]
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Placental alkaline phosphatase/ALPP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for Alkaline Phosphatase/ALPP Antibody (JM22-53)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:400
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody (JM22-53)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (JM22-53)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Alkaline Phosphatase/ALPP Antibody (JM22-53)
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Q: I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
A:
None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.
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