Alpha Actinin 4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89818

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Alpha Actinin 4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22361696)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alpha Actinin 4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody [NBP1-89818]

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody [NBP1-89818]

Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody [NBP1-89818] - Staining of human cell line A-431 shows localization to nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP1-89818]

Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP1-89818]

Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP1-89818] - Staining of human liver shows strong cytoplasmic and membranous positivity in bile duct cells.
Alpha Actinin 4 Antibody - BSA Free Western Blot: Alpha Actinin 4 Antibody - BSA Free [NBP1-89818]

Western Blot: Alpha Actinin 4 Antibody - BSA Free [NBP1-89818]

Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACTN4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Alpha Actinin 4 Antibody - BSA Free Western Blot: Alpha Actinin 4 Antibody - BSA Free [NBP1-89818]

Western Blot: Alpha Actinin 4 Antibody - BSA Free [NBP1-89818]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for Alpha Actinin 4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alpha Actinin 4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Alternate Names

actinin alpha4 isoform, actinin, alpha 4, ACTININ-4, alpha-actinin-4, DKFZp686K23158, F-actin cross-linking protein, focal segmental glomerulosclerosis 1, FSGS, FSGS1, Non-muscle alpha-actinin 4

Gene Symbol

ACTN4

Additional Alpha Actinin 4 Products

Product Documents for Alpha Actinin 4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Alpha Actinin 4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Alpha Actinin 4 Antibody - BSA Free

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Protocols

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