alpha COP I Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89705

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alpha COP I Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VHGNMLHYVKDRFLRQLDFNSSKDVAVMQLRSGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVWVARN

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25894502).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha COP I Antibody - BSA Free

alpha COP I Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: alpha COP I Antibody - BSA Free [NBP1-89705]

Immunocytochemistry/ Immunofluorescence: alpha COP I Antibody - BSA Free [NBP1-89705]

Staining of human cell line A-431 shows positivity in cytoplasm, intermediate filaments & nucleus but excluded from the nucleoli.
Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705]

Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705]

Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705]

Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705]

Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705]

Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705]

Immunohistochemistry-Paraffin: alpha COP I Antibody [NBP1-89705] - Staining of human stomach shows moderate granular cytoplasmic positivity in glandular cells.
alpha COP I Antibody - BSA Free Immunohistochemistry-Paraffin: alpha COP I Antibody - BSA Free [NBP1-89705]

Immunohistochemistry-Paraffin: alpha COP I Antibody - BSA Free [NBP1-89705]

Staining of human pancreas shows moderate to strong granular cytoplasmic positivity in exocrine glandular cells.
alpha COP I Antibody - BSA Free Western Blot: alpha COP I Antibody - BSA Free [NBP1-89705]

Western Blot: alpha COP I Antibody - BSA Free [NBP1-89705]

Analysis in human cell line RT-4.
alpha COP I Antibody - BSA Free Western Blot: alpha COP I Antibody - BSA Free [NBP1-89705]

Western Blot: alpha COP I Antibody - BSA Free [NBP1-89705]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for alpha COP I Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha COP I

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

Alternate Names

alpha coat protein, Alpha-coat protein, Alpha-COP, coatomer protein complex, subunit alpha, coatomer subunit alpha, FLJ26320, HEPCOP, HEP-COPalpha-COP, xenin

Gene Symbol

COPA

Additional alpha COP I Products

Product Documents for alpha COP I Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for alpha COP I Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha COP I Antibody - BSA Free

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Protocols

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