alpha-Fetoprotein/AFP Antibody (OTI2A9)

Novus Biologicals | Catalog # NBP2-03084

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2A9
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

66.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Fetoprotein/AFP Antibody (OTI2A9)

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Alpha 1 Fetoprotein monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Alpha 1 Fetoprotein.
Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Staining of paraffin-embedded Human liver tissue using anti-Alpha 1 Fetoprotein mouse monoclonal antibody.
Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Alpha 1 Fetoprotein antibody, and then analyzed by flow cytometry.
Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha 1 Fetoprotein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha 1 Fetoprotein.
Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084]

Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Analysis of Jurkat cells, using anti-Alpha 1 Fetoprotein antibody, (Red), compared to a nonspecific negative control antibody (Blue).
alpha-Fetoprotein/AFP Antibody (OTI2A9)

alpha-Fetoprotein/AFP Antibody (OTI2A9)[NBP2-03084]

alpha-Fetoprotein/AFP Antibody (OTI2A9)[NBP2-03084]Simple Western� analysis of endogenous protein Adenylosuccinate Lyase (ADSL) from HELA lysates (0.1 mg/mL) using ADSL Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for alpha-Fetoprotein/AFP Antibody (OTI2A9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Fetoprotein/AFP

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq].

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human)

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for alpha-Fetoprotein/AFP Antibody (OTI2A9)

Certificate of Analysis

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Product Specific Notices for alpha-Fetoprotein/AFP Antibody (OTI2A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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