alpha-Internexin Antibody

Novus Biologicals | Catalog # NB300-139

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all alpha-Internexin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant rat alpha internexin expressed in and purified from E. coli. [UniProt# P23565]

Localization

Cytoplasmic

Marker

Immature Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Internexin Antibody

Immunohistochemistry: alpha-Internexin Antibody [NB300-139]

Immunohistochemistry: alpha-Internexin Antibody [NB300-139]

Immunohistochemistry: alpha-Internexin Antibody [NB300-139] - Rat cerebellum section stained with rabbit pAb to alpha-internexin, dilution 1:2,000, in green, and chicken pAb to GFAP, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.
Western Blot: alpha-Internexin Antibody [NB300-139]

Western Blot: alpha-Internexin Antibody [NB300-139]

Western Blot: alpha-Internexin Antibody [NB300-139] - Whole tissue lysates using rabbit pAb to alpha-internexin, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66 kDa range corresponds to alpha-internexin. The alpha-internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.
Western Blot: alpha-Internexin Antibody [NB300-139]

Western Blot: alpha-Internexin Antibody [NB300-139]

alpha-Internexin-Antibody-Western-Blot-NB300-139-img0008.jpg
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB300-139]

Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB300-139]

Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - IF Confocal analysis of SHSY5Y cells using alpha Internexin antibody (NB300-139, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Western Blot: alpha-Internexin Antibody [NB300-139]

Western Blot: alpha-Internexin Antibody [NB300-139]

Western Blot: alpha Internexin Antibody [NB300-139] - Western blot of whole rat spinal cord homogenate stained with RPCA-a-Int, at dilution of 1:20,000. A prominent band running at ~66kDa is apparent, as well as smaller lower bands which are apparently degradation products. A minor band at ~150kDa is also seen, apparently resulting from dimerization of alpha-internexin.
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB300-139]

Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB300-139]

Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin CPCA-Peri (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons.
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB300-139]

Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB300-139]

Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - Polyclonal alpha internexin, NB 300-139 (red) staining neuronal progenitor cells. The green stain shows the fibroblast marker, Plectin (not one of our antibodies).
alpha-Internexin Antibody

Western Blot: alpha-Internexin Antibody [NB300-139] -

Proteins involved in morphogenesis.The expression of a-internexin (INA), collapsing response mediator protein 2 (CRMP2) and 4 (CRMP4), neurofilament-low (NFL) and tubuline-b2 (Tub beta 2) were analyzed by Western blotting. White bars are controls, grey bars are enucleated and black bars are anophthalmic mice. Results from V1 (left), V2M (middle) and V2L (right) are shown for each protein. * P < 0.05 and ** P < 0.01.
alpha-Internexin Antibody

Western Blot: alpha-Internexin Antibody [NB300-139] -

Western Blot: alpha-Internexin Antibody [NB300-139] - Proteins involved in morphogenesis.The expression of a-internexin (INA), collapsing response mediator protein 2 (CRMP2) & 4 (CRMP4), neurofilament-low (NFL) & tubuline-b2 (Tub beta 2) were analyzed by Western blotting. White bars are controls, grey bars are enucleated & black bars are anophthalmic mice. Results from V1 (left), V2M (middle) & V2L (right) are shown for each protein. * P < 0.05 & ** P < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27410964), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for alpha-Internexin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

1:500 - 1:1000

Western Blot

1:10000 - 1:20000
Application Notes
This alpha Internexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where a band is observed ~ 66kDa. Use in IP was reported in the scientific literature (PMID: 23802559).

Reviewed Applications

Read 1 review rated 5 using NB300-139 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Supplied as serum

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Internexin

In mammalian CNS, five key neuronal intermediate filament (NIF) proteins have been discovered which includes alpha-internexin (66 kD, INA or Inexa), peripherin (57 kD), and three neurofilament (NF) proteins, which are neurofilament light (NF-L, 68 kD), medium (NF-M, 145 kD), and heavy (NF-H, 200 kD). Among NIFs, alpha-internexin is categorized as class IV NIF which is highly expressed in most neurons during development especially when they begin to differentiate and before the expression of NF triplet proteins, and it is structurally/functionally associated with NF triplet proteins inside mature CNS neurons. Peripherin is mainly expressed in PNS and in some CNS neurons also, and alpha-internexin - peripherin self-assemble or co-assemble with neurofilament protein subunits to form filamentous structure before their translocation into the axons to become an essential constituent of shape-maintaining IF network in mature neurons. It has been recognized as one of the many proteins phosphorylated by ATM/ATR in response to DNA damage and has been associated with developmental, degenerative /overexpression causes neuronal cell death, and inflammatory processes of CNS. Abnormal NIF accumulation is a neuropathological signature of several neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, dementia with Lewy bodies, and amyotrophic lateral sclerosis. Alpha-internexin has been detected as a major component of pathological inclusions in frontotemporal dementia, also called 'neuronal intermediate filament inclusion disease (NIFID).

Alternate Names

alphaInternexin, INA, NEF5, NF-66, TXBP-1

Entrez Gene IDs

9118 (Human); 24503 (Rat)

Gene Symbol

INA

UniProt

P23565 (Rat)

Additional alpha-Internexin Products

Product Documents for alpha-Internexin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for alpha-Internexin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Internexin Antibody

Customer Reviews for alpha-Internexin Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used alpha-Internexin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/16/2014
    IF Confocal analysis of SHSY5Y cells using alpha Internexin antibody (NB300-139, 1:5).
    alpha-Internexin Antibody NB300-139

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies