alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43875

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9360

Format

Azide and BSA Free
View all alpha-Methylacyl-CoA Racemase/AMACR Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q9UHK6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Analysis in human kidney and skeletal muscle tissues using NBP3-43875 antibody. Corresponding alpha-Methylacyl-CoA Racemase/AMACR RNA-seq data are presented for the same tissues.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Staining of human liver cancer shows moderate granular cytoplasmic positivity in tumor cells.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Staining of human kidney shows strong granular cytoplasmic positivity in cells in proximal tubules.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Staining of human pancreas shows weak granular cytoplasmic positivity in islets of Langerhans.
alpha-Methylacyl-CoA Racemase/AMACR Antibody (CL9360) - Azide and BSA Free Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody [NBP3-43875]

Analysis in human cell line CACO-2.

Applications

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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