alpha Tubulin Antibody (TU-01) - BSA Free

Novus Biologicals | Catalog # NB500-333

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, A. thaliana, Eukaryote, Invertebrate, Plant, Turkey, Yeast

Cited:

Plant

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TU-01

Format

BSA Free
View all alpha Tubulin Primary Antibodies »

Product Specifications

Immunogen

This alpha Tubulin Antibody (TU-01) was developed against a fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization.

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 26594217).

Localization

Major constituent of microtubules.

Marker

Microtubule Marker

Specificity

This alpha Tubulin Antibody (TU-01) recognizes the defined epitope (aa 65-97) on N-terminal structureal domain of alpha tubulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Tubulin Antibody (TU-01) - BSA Free

Western Blot: alpha Tubulin Antibody (TU-01)BSA Free [NB500-333]

Western Blot: alpha Tubulin Antibody (TU-01)BSA Free [NB500-333]

Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody; alpha tubulin molecular weight: 50 kDa. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) and anti-Vimentin (VI-01; red). Nucleus is stained with DAPI (blue).
Immunoprecipitation: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunoprecipitation: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunoprecipitation: alpha Tubulin Antibody (TU-01) [NB500-333] - alpha Tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Molecular weight of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions) IPn = immunoprecipitate (non-reducing conditions)
Western Blot: alpha Tubulin Antibody (TU-01)BSA Free [NB500-333]

Western Blot: alpha Tubulin Antibody (TU-01)BSA Free [NB500-333]

Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of alpha-tubulin (molecular weight of 50 kDa) in porcine brain lysate by antibody TU-01 biotin.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Tubulin filament in HeLa. Image from verified customer review.
Western Blot: alpha Tubulin Antibody (TU-01)BSA Free [NB500-333]

Western Blot: alpha Tubulin Antibody (TU-01)BSA Free [NB500-333]

Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of lysates of various cell lines by a newly developed monoclonal antibody (B). Alpha tubulin molecular weight: 50 kDa.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue).
alpha Tubulin Antibody (TU-01) - BSA Free

Western Blot: alpha Tubulin Antibody (TU-01) - BSA Free [NB500-333] -

Exo70 is redistributed into PSD in the hippocampus of mTBI mice. a Schematic representation of subcellular fractionation proteins. b Example of subcellular fractionation. Hippocampus from two-month-old male mice was fractionated and microsome, PSD, and nonPSD fractions were obtained. 20 ug of protein samples were resolved in a 10% SDS-PAGE and transferred to PVDF membranes. Membranes were incubated with the respective antibodies shown in the figure. Membranes were stripped and tested again with the indicated antibodies. c Proteins distribution were analyzed with densitometric analysis by comparing signal intensity from each fraction with homogenized. Mean values +/- SEM are shown. d Cortex and Hippocampus (f) from Sham and mTBI mice were fractionated and analyzed by western blot using Exo70, PDI, Actin, and Tubulin antibodies. PDI/Actin/Tubulin was used as loading controls. 30 ug of protein samples were used. e, g The graph shows the Exo70 densitometric analysis normalized with loading controls. Values represent means +/- SEM, n = 3 mice per experimental group. Statistical differences were determined by an unpaired t-test comparing Sham and mTBI. *p < 0.05, **p < 0.01 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33593425), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for alpha Tubulin Antibody (TU-01) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1-2 ug/ml
Application Notes
Western blotting: Recommended dilution in reducing conditions.

Reviewed Applications

Read 1 review rated 5 using NB500-333 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: alpha Tubulin

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T.,... Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Long Name

Tubulin Alpha 1a

Alternate Names

Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1

Entrez Gene IDs

20766 (Mouse)

Gene Symbol

TUBA1A

UniProt

P02550 (Porcine)

Additional alpha Tubulin Products

Product Documents for alpha Tubulin Antibody (TU-01) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for alpha Tubulin Antibody (TU-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Microtubules

Citations for alpha Tubulin Antibody (TU-01) - BSA Free

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293T and NIH3T3 whole cell lysate
    Species: Human
    Verified Customer | Posted 03/15/2016
    Tubulin Antibody (NB500-333)
    alpha Tubulin Antibody (TU-01) - BSA Free NB500-333

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Protocols

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FAQs for alpha Tubulin Antibody (TU-01) - BSA Free

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  • Q: We would like to stain cilia with an acetylated alpha tubulin antibody in our cells, but I am unsure if this antibody will be able to conclusively differentiate cilia from other structures such as spindle pole bodies. Does anyone know what acetylated alpha tubulin antibodies might bind to apart from cilia?

    A: Acetylated alpha tubulin is found in relatively stable microtubules. It is best practice to use this marker together with a centrosome/centriole marker, which will stain the basal bodies at the base of the cilium. After that, it is relatively straightforward to identify the acetylated alpha tubulin signal that corresponds to the cilium.

  • Q: Will this alpha tubulin antibody recognize both isoforms of alpha tubulin? 

    A: The epitope for this alpha tubulin antibody lies on the C-terminus of the protein and the difference between the two major isoforms is within the first 35 aa of the N-terminus so this alpha tubulin antibody will recognize both isoforms.

  • Q: We would like to stain cilia with an acetylated alpha tubulin antibody in our cells, but I am unsure if this antibody will be able to conclusively differentiate cilia from other structures such as spindle pole bodies. Does anyone know what acetylated alpha tubulin antibodies might bind to apart from cilia?

    A: Acetylated alpha tubulin is found in relatively stable microtubules. It is best practice to use this marker together with a centrosome/centriole marker, which will stain the basal bodies at the base of the cilium. After that, it is relatively straightforward to identify the acetylated alpha tubulin signal that corresponds to the cilium.

  • Q: Will this alpha tubulin antibody recognize both isoforms of alpha tubulin? 

    A: The epitope for this alpha tubulin antibody lies on the C-terminus of the protein and the difference between the two major isoforms is within the first 35 aa of the N-terminus so this alpha tubulin antibody will recognize both isoforms.

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