Key Product Details

Species Reactivity

Human, Rat, Bovine

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A10.C9

Format

BSA Free
View all AlphaB Crystallin/CRYAB Primary Antibodies »

Product Specifications

Immunogen

Native Alpha B Crystallin

Localization

Cytoplasm, Nucleus

Specificity

Detects approx 20kDa (predicted mol.weight is approx 21kDa). Does not cross-react with alpha A-crystallin, Beta L-crystallin, Beta H-crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (3A10.C9) - BSA Free

Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410]

Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410]

Western Blot: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410]

Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410]

Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (3A10.C9) [NBP1-22410] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X.

Applications for AlphaB Crystallin/CRYAB Antibody (3A10.C9) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Western Blot

1:2000
Application Notes
0.5 ug/ml of Alpha B Crystallin Antibody was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alphacrystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is over expressed in several neurological disorders and in cell lines under stress conditions (9).

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Entrez Gene IDs

1410 (Human)

Gene Symbol

CRYAB

UniProt

NP_001876.1 (Human), P02511 (Human)

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB Antibody (3A10.C9) - BSA Free

Certificate of Analysis

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Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (3A10.C9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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