AlphaB Crystallin/CRYAB Antibody (CL10758) - BSA Free

Novus Biologicals | Catalog # NBP3-21180

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10758

Format

BSA Free
View all AlphaB Crystallin/CRYAB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P02511, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse AlphaB Crystallin/CRYAB Antibody (CL10758) - BSA Free (NBP3-21180) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (CL10758) - BSA Free

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Staining of human cerebellum shows strong cytoplasmic positivity in oligodendrocytes.
Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Staining of U-138 MG cells using the Anti-CRYAB monoclonal antibody, showing specific staining in nuclear speckles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Analysis in human cerebral cortex and liver tissues using NBP3-21180 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.
Western Blot: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Western Blot: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Analysis in human cell line U-2 OS.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglia and nerves.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Staining of human cerebral cortex shows strong cytoplasmic positivity in oligodendrocytes.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP3-21180] -

Staining of rat brain shows strong cytoplasmic positivity in oligodendrocytes.

Applications for AlphaB Crystallin/CRYAB Antibody (CL10758) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20000 - 1:50000

Immunohistochemistry-Paraffin

1:20000 - 1:50000

Western Blot

1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Gene Symbol

CRYAB

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB Antibody (CL10758) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (CL10758) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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