AlphaB Crystallin/CRYAB Antibody (OTI6A9)

Novus Biologicals | Catalog # NBP1-47708

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6A9
View all AlphaB Crystallin/CRYAB Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens crystallin, alpha B (CRYAB).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP1-47708) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-AlphaB Crystallin/CRYAB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (OTI6A9)

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Crystallin AB.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human pancreas tissue using anti-Crystallin AB mouse monoclonal antibody.
Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Crystallin AB antibody, and then analyzed by flow cytometry.
Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of HT29 cells using anti-Crystallin AB mouse monoclonal antibody.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human endometrium tissue using anti-Crystallin AB mouse monoclonal antibody.
Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708]

Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
AlphaB Crystallin/CRYAB Antibody (OTI6A9)

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] -

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).

Applications for AlphaB Crystallin/CRYAB Antibody (OTI6A9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:500

Reviewed Applications

Read 1 review rated 5 using NBP1-47708 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development,these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Entrez Gene IDs

1410 (Human)

Gene Symbol

CRYAB

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB Antibody (OTI6A9)

Certificate of Analysis

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Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (OTI6A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AlphaB Crystallin/CRYAB Antibody (OTI6A9)

Customer Reviews for AlphaB Crystallin/CRYAB Antibody (OTI6A9) (1)

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  • AlphaB Crystallin/CRYAB Antibody (OTI6A9)
    Name: Anonymous
    Application: Immunoflurescence
    Sample Tested: muscle tissue
    Species: Mouse
    Verified Customer | Posted 08/17/2021
    AlphaB Crystallin/CRYAB Antibody in muscle
    AlphaB Crystallin/CRYAB Antibody (OTI6A9) NBP1-47708

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