Aly Antibody (11G5) - BSA Free

Novus Biologicals | Catalog # NB100-670

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Xenopus

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 11G5

Format

BSA Free
View all Aly Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ALY / REF protein

Reactivity Notes

Cross-reacts with Human and Xenopus laevis. Not yet tested in other species.

Localization

Nuclear

Specificity

This is specific for Aly protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Aly Antibody (11G5) - BSA Free

Western Blot: Aly Antibody (11G5)BSA Free [NB100-670]

Western Blot: Aly Antibody (11G5)BSA Free [NB100-670]

Western Blot: Aly Antibody (11G5) [NB100-670] - La cell extract was separated by SDS-PAGE and probed with Monoclonal Anti-ALY (0.4 ug/mL, lane 1) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304, lane 1,2) and a chemiluminescent substrate.

Applications for Aly Antibody (11G5) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

0.5-1 ug/ml
Application Notes
Co-immunoprecipitation and western blot studies were performed in Hela cells using a monoclonal mouse anti-Aly antibody. The results indicated that Aly which was observed as a 30 kDa band interacted with the Herpes Simplex Virus I protein, ICP27. 1

Reviewed Applications

Read 1 review rated 5 using NB100-670 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

2.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aly

Pre-mRNA splicing plays an important role in regulation of gene expression. During the splicing process, specific proteins are recruited to the mRNA and assembled into a complex of mRNA-protein near the exon-exon junction. This complex is termed the mRNA-protein complex (mRNP) or the exon-exon junction complex. The proteins that are found in this complex include: Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK.1-5 Aly (also known as mREF1-I) is the mammalian homologue of the yeast mRNA export factor Yra1p.6-7 It is a 233 amino acid RNAbinding protein that contains a RNA-binding domain and is recruited to the mRNP complexes during the splicing. Excess of recombinant expression of Aly in the cells increases both the rate and efficiency of spliced and unspliced mRNA export (in vivo) from the nucleus to the cytoplasm. In contrast to the Y14 protein, Aly is dissociated from the mRNAs after the export to the cytoplasm.1, 5-6

Alternate Names

Ally of AML-1 and LEF-1, ALY/REF, ALYBEFbZIP enhancing factor, bZIP-enhancing factor BEF, REF, THO complex 4, THO complex subunit 4, Tho4, Transcriptional coactivator Aly/REF

Entrez Gene IDs

10189 (Human)

Gene Symbol

ALYREF

UniProt

Q86V81 (Human)

Additional Aly Products

Product Documents for Aly Antibody (11G5) - BSA Free

Certificate of Analysis

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Product Specific Notices for Aly Antibody (11G5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aly Antibody (11G5) - BSA Free

Customer Reviews for Aly Antibody (11G5) - BSA Free (1)

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  • Aly Antibody (11G5) - BSA Free
    Name: Masashi Okada
    Application: Western Blot
    Sample Tested: 293 cell extract and 293T cell line
    Species: Human
    Verified Customer | Posted 07/15/2023
    293 cells transiently transfected siALY or siCt for 48h, then 10 ug of cell lysates were separated by SDS-PAGE and detected WB. Ref: Proc Natl Acad Sci U S A. 2008 Jun 24;105:8649-54.
    Aly Antibody (11G5) - BSA Free NB100-670

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