Aly Antibody (HL3813)

Novus Biologicals | Catalog # NBP3-48848

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3813
View all Aly Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Aly.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Aly Antibody (HL3813) (NBP3-48848) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Aly Antibody (HL3813)

Aly Antibody (HL3813)

Western Blot: Aly Antibody (HL3813) [NBP3-48848] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aly antibody [HL3813] (NBP3-48848) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Aly Antibody (HL3813)

Western Blot: Aly Antibody (HL3813) [NBP3-48848] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aly antibody [HL3813] (NBP3-48848) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Aly Antibody (HL3813)

Immunohistochemistry-Paraffin: Aly Antibody (HL3813) [NBP3-48848] -

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
Aly Antibody (HL3813)

Immunohistochemistry-Paraffin: Aly Antibody (HL3813) [NBP3-48848] -

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
Aly Antibody (HL3813)

Immunohistochemistry-Paraffin: Aly Antibody (HL3813) [NBP3-48848] -

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
Aly Antibody (HL3813)

Immunohistochemistry-Paraffin: Aly Antibody (HL3813) [NBP3-48848] -

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
Aly Antibody (HL3813)

Immunohistochemistry-Paraffin: Aly Antibody (HL3813) [NBP3-48848] -

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
Aly Antibody (HL3813)

Immunohistochemistry-Paraffin: Aly Antibody (HL3813) [NBP3-48848] -

Aly antibody [HL3813] detects Aly protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Aly stained by Aly antibody [HL3813] (NBP3-48848) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Applications for Aly Antibody (HL3813)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aly

Pre-mRNA splicing plays an important role in regulation of gene expression. During the splicing process, specific proteins are recruited to the mRNA and assembled into a complex of mRNA-protein near the exon-exon junction. This complex is termed the mRNA-protein complex (mRNP) or the exon-exon junction complex. The proteins that are found in this complex include: Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK.1-5 Aly (also known as mREF1-I) is the mammalian homologue of the yeast mRNA export factor Yra1p.6-7 It is a 233 amino acid RNAbinding protein that contains a RNA-binding domain and is recruited to the mRNP complexes during the splicing. Excess of recombinant expression of Aly in the cells increases both the rate and efficiency of spliced and unspliced mRNA export (in vivo) from the nucleus to the cytoplasm. In contrast to the Y14 protein, Aly is dissociated from the mRNAs after the export to the cytoplasm.1, 5-6

Alternate Names

Ally of AML-1 and LEF-1, ALY/REF, ALYBEFbZIP enhancing factor, bZIP-enhancing factor BEF, REF, THO complex 4, THO complex subunit 4, Tho4, Transcriptional coactivator Aly/REF

Gene Symbol

ALYREF

Additional Aly Products

Product Documents for Aly Antibody (HL3813)

Certificate of Analysis

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Download SDS

Product Specific Notices for Aly Antibody (HL3813)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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