![Western Blot: AMID AntibodyBSA Free [NBP3-18132]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Western-Blot-NBP3-18132-img0003.jpg "Western Blot: AMID AntibodyBSA Free [NBP3-18132]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (92%), Chimpanzee (100%), Equine (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AMID. The immunogen is located within the last 50 amino acids of AMID.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AMID Antibody - BSA Free
Western Blot: AMID AntibodyBSA Free [NBP3-18132]
Western Blot: AMID Antibody - BSA Free [NBP3-18132] - WB Validation in Rat Tissues. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18132, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.![Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18132-img0006.jpg "Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]")
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-AMID antibody (NBP3-18132) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.![Western Blot: AMID AntibodyBSA Free [NBP3-18132]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Western-Blot-NBP3-18132-img0001.jpg "Western Blot: AMID AntibodyBSA Free [NBP3-18132]")
Western Blot: AMID AntibodyBSA Free [NBP3-18132]
Western Blot: AMID Antibody - BSA Free [NBP3-18132] - WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18132, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.![Western Blot: AMID AntibodyBSA Free [NBP3-18132]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Western-Blot-NBP3-18132-img0002.jpg "Western Blot: AMID AntibodyBSA Free [NBP3-18132]")
Western Blot: AMID AntibodyBSA Free [NBP3-18132]
Western Blot: AMID Antibody - BSA Free [NBP3-18132] - WB Validation in Mouse Tissues. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18132, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.![Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18132-img0005.jpg "Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]")
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132] - Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-AMID antibody (NBP3-18132) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.![Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]](https://resources.rndsystems.com/images/products/AMID Antibody - BSA Free-Immunohistochemistry-Paraffin-NBP3-18132-img0007.jpg "Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]")
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132]
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AMID antibody (NBP3-18132) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: AMID Antibody - BSA Free [NBP3-18132] -
Immunocytochemistry/ Immunofluorescence: AMID Antibody - BSA Free [NBP3-18132] -Validation of AIFM2 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).Applications for AMID Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
1 ug/ml
Western Blot
2 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AMID
Alternate Names
AMID, apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death, apoptosis-inducing factor 2, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, apoptosis-inducing factor, mitochondrion-associated, 2, Apoptosis-inducing factor-like mitochondrion-associated inducer of death, DKFZp686L1298, FLJ14497, p53-responsive gene 3 protein, PRG3apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer ofdeath, RP11-367H5.2
Gene Symbol
AIFM2
Additional AMID Products
Product Documents for AMID Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AMID Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for AMID Antibody - BSA Free
There are currently no reviews for this product. Be the first to review AMID Antibody - BSA Free and earn rewards!
Have you used AMID Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...