![Western Blot: AMID AntibodyBSA Free [NBP3-18133]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Western-Blot-NBP3-18133-img0007.jpg "Western Blot: AMID AntibodyBSA Free [NBP3-18133]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Chimpanzee (100%), Equine (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the center of human AMID. The immunogen is located within 130-180 amino acids of AMID.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit AMID Antibody - BSA Free (NBP3-18133) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for AMID Antibody - BSA Free
Western Blot: AMID AntibodyBSA Free [NBP3-18133]
Western Blot: AMID Antibody - BSA Free [NBP3-18133] - WB Validation in Mouse Skeletal Muscle. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18133, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.![Immunocytochemistry/ Immunofluorescence: AMID Antibody - BSA Free [NBP3-18133]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-18133-img0003.jpg "Immunocytochemistry/ Immunofluorescence: AMID Antibody - BSA Free [NBP3-18133]")
Immunocytochemistry/ Immunofluorescence: AMID Antibody - BSA Free [NBP3-18133]
Immunocytochemistry/Immunofluorescence: AMID Antibody - BSA Free [NBP3-18133] - Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AMID with NBP3-18133 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).![Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18133-img0005.jpg "Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]")
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AMID antibody (NBP3-18133) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.![Western Blot: AMID AntibodyBSA Free [NBP3-18133]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Western-Blot-NBP3-18133-img0001.jpg "Western Blot: AMID AntibodyBSA Free [NBP3-18133]")
Western Blot: AMID AntibodyBSA Free [NBP3-18133]
Western Blot: AMID Antibody - BSA Free [NBP3-18133] - WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18133, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.![Western Blot: AMID AntibodyBSA Free [NBP3-18133]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Western-Blot-NBP3-18133-img0002.jpg "Western Blot: AMID AntibodyBSA Free [NBP3-18133]")
Western Blot: AMID AntibodyBSA Free [NBP3-18133]
Western Blot: AMID Antibody - BSA Free [NBP3-18133] - WB Validation in Rat Tissues. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18133, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.![Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]](https://resources.rndsystems.com/images/products/AMID-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18133-img0004.jpg "Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]")
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133] - Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-AMID antibody (NBP3-18133) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.![Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]](https://resources.rndsystems.com/images/products/AMID Antibody - BSA Free-Immunohistochemistry-Paraffin-NBP3-18133-img0006.jpg "Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]")
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133]
Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18133] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AMID antibody (NBP3-18133) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.Applications for AMID Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
1 ug/ml
Western Blot
2 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AMID
Alternate Names
AMID, apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death, apoptosis-inducing factor 2, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, apoptosis-inducing factor, mitochondrion-associated, 2, Apoptosis-inducing factor-like mitochondrion-associated inducer of death, DKFZp686L1298, FLJ14497, p53-responsive gene 3 protein, PRG3apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer ofdeath, RP11-367H5.2
Gene Symbol
AIFM2
Additional AMID Products
Product Documents for AMID Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for AMID Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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