Aminoacylase/ACY1 Antibody (4F1-B7) - Azide and BSA Free

Novus Biologicals | Catalog # H00000095-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F1-B7

Format

Azide and BSA Free
View all Aminoacylase/ACY1 Primary Antibodies »

Product Specifications

Immunogen

ACY1 (AAH00545, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTSKGPAEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDVKCVSIQYLEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCKDMNLTLEPEIMPAATDNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSDS

Specificity

ACY1 - aminoacylase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Aminoacylase/ACY1 Antibody (4F1-B7) - Azide and BSA Free

Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody (M01), clone 4F1-B7.Lane 1: ACY1 transfected lysate(45.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Immunocytochemistry/ Immunofluorescence: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Immunocytochemistry/Immunofluorescence: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of monoclonal antibody to ACY1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Immunohistochemistry-Paraffin: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 3 ug/ml.
Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Western Blot: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - ACY1 monoclonal antibody (M01), clone 4F1-B7. Analysis of ACY1 expression in K-562.
Immunoprecipitation: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Immunoprecipitation: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01]

Immunoprecipitation: Aminoacylase/ACY1 Antibody (4F1-B7) [H00000095-M01] - Analysis of ACY1 transfected lysate using anti-ACY1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACY1 MaxPab rabbit polyclonal antibody.

Applications for Aminoacylase/ACY1 Antibody (4F1-B7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Aminoacylase/ACY1

Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Alternate Names

ACY1

Entrez Gene IDs

95 (Human)

Gene Symbol

ACY1

OMIM

104620 (Human)

UniProt

AAH00545 (Human)

Additional Aminoacylase/ACY1 Products

Product Documents for Aminoacylase/ACY1 Antibody (4F1-B7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Aminoacylase/ACY1 Antibody (4F1-B7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

Citations for Aminoacylase/ACY1 Antibody (4F1-B7) - Azide and BSA Free

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Protocols

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FAQs

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